WIKI资讯
WIKI资讯
Cellular Transfection and Immunoprecipitation Before proceeding with the experiments outlined below, all kinase pocket mutants should be characterized for their expression, localization, specific activity, and substrate specificity. The results should be compared with those obtained from the wild-type kinase to ensure that the mutation does not significantly alter these characteristics.Grow COS-1 cells in ......阅读全文
Identification of a Mutant Kinase/ATP Analog PairScott T. Eblen, N. Vinay Kumar, and Michael J. WeberDepartment of Microbiology and Cancer Center, Uni