WIKI资讯
WIKI资讯
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of cell adhesion under well-defined wall shear stress. The visualization of the different events of cell adhesion can be quantified by selective image acquisition and subsequent image processing. Flow assays are uniquely suited to the investigation of adhesive events which ......阅读全文
Cell Adherence Assay (LTI)General and nice Protocol for cell adherence assay. Proteins are coated on microtiter plates and cells are added; after
Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a gi
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
PurposeThe main purpose of the flow chamber assay is to visualize and measure interactions between flowing cells expressing a given adhesion molecule
BASIC THEORY AND USE OF GC-MSbyDr. Eugenia SobolevaContent1. Introduction.2. GC-MS syste
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
Sequencing GelPreparing and Running a Sequencing Gel (6% Polyacrylamide/Urea) A. Preparation of Gel Solution 1) Weigh out 50 g of Urea into
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
2013年5月16日-19日，由中国化学会主办、厦门大学承办、复旦大学、浙江大学协办的为期四天的第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议在美丽的海滨城市厦门隆重召开。以下是微全分析专场报告。上海交通大学
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Cytokine ELISPOT ProtocolDescriptioneBioscience ELISPOT Ready-SET-Go! reagent sets contain the necessary reagents for performing enzyme linked immunos
1. Introduction and BackgroundThere is a great need for general methods to characterize the proteins that contemporary biology makes available. The li
Full size image (70 KB)In vitro incubation of a reaction mix that contains labeled endosomes, cytosol and an ATP-regenerating system at phys
实验概要The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
实验概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
自动化TPS系列是中型开放式自动化移液工作站。一起自动化程度更高，自动更换吸头，轻松助您完成高倍率梯度稀释，单针挑样、均一化，96/384/1536孔板等移液操作，并可整合振荡器、孵育模块等功能模块。以下是美杏高德使用TPS结合微孔板筛查生物素酶的缺陷实验报告，请参考：INTRODUCTIONBio
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
cAMP AssaysGouzel Karimova and Daniel LadantUnite Postulante de Biochimie des Interactions Macromoleculaires, Departement de Biologie Structurale et C
21. Add 100 µL of developing buffer to all wells using a multichannel pipette and incubate at room temperature for at least 2
Springer Lab，The CBR Institute for Biomedical Research, Inc. Department of Pathology Harvard Medical Schoolhttp://cbr.med.harvard.edu/investigato
PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cel
eBioscience公司Luminex液相蛋白芯片检测试剂——ProcartaPlex可以为您的生物学研究提供各种多因子检测方案，包括细胞因子、生长因子、趋化因子和其他蛋白。在选择试剂盒及操作时会遇到很多问题，现将常见问题及答复汇总如下，供您参考： 如果不小心将整个试剂盒保存在-20°C. 这个试
1、Required Materials and Equipment(1) Protein A or G agarose gel column (10 ml or 5 ml of packed beads; see guidelines below for choice of protein A o
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the
MATERIALS:2-glass plates1 sharks -tooth comb and spacersWhatman 3 mm paper30 or 40% acrylamide-bis (19:1)10X TBEurea10% ammonium persulfateTEMED60 cc.
Spore Prep - Inoculating & HarvestingDescription A spore prep is a method of preserving a sporulating strain of Streptomyces. The stock is st
Figure 1: epMotion 5073l worktable for qPCR setup Results and DiscussionThe automation of the qPCR setup using the 10 µL dispensing tool a
Maintenance of Cell CultureAuthor: Nanci DonackiSource: Contributed by Nanci DonackiDate Added: Tue May 14 2002Date Modified: Tue
Intergeneric Conjugation and OverlayDescription Transfer of plasmid/cosmid DNA from a host strain, e.g. E.coli ET12567 [pUZ8002], to the recipien
The ELISA protocols for detection of the antibody binding to an antigen-coated microtitre plate are standard laboratory techniques and will not be des