WIKI资讯
WIKI资讯
Eukaryotic messenger RNA (mRNA) can be separated from the other RNA species in a total RNA preparation by affinity chromatography by virtue of the presence of a polyadenylic acid “tail,” 20–25 bases long, at the 3′ end of the molecule (1). Oligo(dT)-cellulose is used routinely for homemade affinity columns, but a range of ready-made products is now available from a number of suppliers (e.g., Hybond-mAp, A......阅读全文
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
实验概要E.Z.N.A.® Total RNA Midiprep Kit provides a rapid and easy method for the isolation of up to 600 ug of total RNA from cultured eukaryo
AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are de
实验概要E.Z.N.A.® Total RNA Midiprep Kit provides a rapid and easy method for the isolation of up to 600 ug of total RNA from cultured eukaryo
实验概要The SuperScript™ III One-Step RT-PCR System with Platinum® Taq High Fidelity is designed for sensitive, high-fidelity end-p
实验概要E.Z.N.A.™ MicroElute® Total RNA Kit provides a rapid and easy method for the isolation of up to 50 ug of total RNA from small amount o
This method was submitted by Jim Hutchins from the University of Mississippi Medical Center.The following is a great RT- PCR protocol develo
This method was submitted by Jim Hutchins from the University of Mississippi Medical Center.The following is a great RT-PCR protocol developed by
主要内容如下：· RT-PCR· Competitive and Quantative
cDNA LibrariesIsolation of corresponding genetic informationInstead of synthesizing a desired gene, can we used the amino acid information to directly
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Cor
泛抗冠状病毒药物三类靶点 非结构型蛋白：3c样蛋白酶（3CLpro）、木瓜蛋白酶（PLpro）和RNA依赖性RNA聚合酶（RdRp）。结构性蛋白：Spike（S）糖蛋白、包膜（E）蛋白、膜（M）蛋白和核帽（N）蛋白辅助蛋白：病毒间变异太大，不是很好的药物靶点靶向CoV非结构性蛋白（nons