ProtocolsforLCMpreparationandanalysis
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Preparation and LCM of Paraffin Embedded Tissue SectionsFor both I & II Frozen & Paraffin-EmbeddedTissue SectionsD. Laser TransferE. DNA Extraction Protocol of LCM tissue and PCR for DNA analysisF. RNA Extraction Protocol of LCM ......阅读全文
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Pr
LCM－PROTOCOLS
Slide Sectioning Paraffin blocks- For DNA analysis: Special LCM processing schedule is followed. The water bath is cleaned using RNAse Zap™, rinse
Preparation－Of－Peripheral－Blood－Cells－For－Chromosome－Analysis
实验概要 Lymphocytes are differentiated cells which normally do not undergo subsequent cell divisions. By culturing lymphocytes in the presence of a m
Preparation－of－denaturing－6％polyacrylamide－gels－for－microsatellite－analysis
Preparation of denaturing 6% polyacrylamide gels for microsatellite analysis(also for SSAP, high-resolution IRAP/ISSR and other analyses)Saadiah Jamli
Preparation－of－Bacterial－Proteins－for－Analysis－by－2DPAGE
The following protocol has been developed for preparing soluble bacterial proteins in a form suitable for analysis by 2D-PAGE. The procedure was prin
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
组织学——显微解剖
Laser Capture Microdissection (LCM)Introduction to LCM (BJMU) Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections (NIH Laser Capture M
细胞遗传学——染色体
Chromosome Staining and Banding Technique (Primate Cytogenetics Network)Protocols for different staining method, each is in great detail. Karyotype A
DNA微序列技术
· Protocols for Making Drosophila Arrays (Stanford U.)Detailed protocol for making arrays including PCR Amplification of cDNAs for Printing,
组织学——组织制备
· Histological techniques (William H. Heidcamp)Very detailed guide to histological techniques, like fixation, dehydration, embedment and subs
LCM－实验
试剂、试剂盒 RNaseAway仪器、耗材 胶带LCM 棺子去湿器分液器镊子LCM 系统 Pix Cell Ⅱ无 RNA 酶的管子实验步骤 一、材料1. 缓冲液、溶液和试剂RNaseAway(7000，MolecularBioProducts，SanDiego，CA)2. 特殊设备.胶带LCM 棺子
LCM－实验
LCM实验对（1）切片的病理分析（2）病变程度的分析（3）临床样本收集与参考等都有应用。实验方法原理采用激光切割等技术将生物样品片子等不易观察和检测的样品进行处理的技术，具有简单快捷，效率高等特点。实验材料RNaseAway试剂、试剂盒RNaseAway仪器、耗材胶带LCM 棺子去湿器分液器镊子LC
LCM－实验
实验方法原理 采用激光切割等技术将生物样品片子等不易观察和检测的样品进行处理的技术，具有简单快捷，效率高等特点。 实验材料 RNaseAway
多克隆抗体
Making Antibody· Production of Polyclonal Antibody in Rabbit (Walter Steffen)Provides detailed protocol for immunizatioin, bleeding procedure,
细胞组分和细胞器——细胞器分离
Labeling Microtubules (Molecular Dynamics Inc. )Microtubules are involved in many aspects of cell motion including propulsion, mitosis, growth, and o
LCM显微切割
这段时间做的LCM显微切割少量组织，提取ng量级RNA的过程奉上。此类RNA可以经两轮扩增后上样进行基因芯片的研究；或直接作为模板进行RT-PCR的研究。虽然过程复杂一些，目前看效果还是不错。 标本来自新鲜的动物或人体的组织标本，取材后液氮速冻后，－80℃存放备用。 标本冰冻切片的要求：
LCM显微切割
这段时间做的LCM显微切割少量组织，提取ng量级RNA的过程奉上。此类RNA可以经两轮扩增后上样进行基因芯片的研究；或直接作为模板进行RT-PCR的研究。虽然过程复杂一些，目前看效果还是不错。 标本来自新鲜的动物或人体的组织标本，取材后液氮速冻后，－80℃存放备用。 标本冰冻切片的要求： 1.每例标
流式细胞仪技术专辑
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excitation
GenomeWide－Identification－of－Transcription－FactorBinding－Sites－in...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip)
Horizon和Waters公司推出水分析的新型样品制备技术
Oasis® HLB萃取盘为环境实验室创造了更高的效率 简介：Salem, NH, 2008年11月3日—— Horizon技术公司和Waters公司推出了Oasis HLB萃取盘，一种用于EPA 8270和1694方法分析的固相萃取产品。 Horizon Technology and Wate
酵母遗传学技术
Genome-wide Gene Expression Analysis (Richard Young Research Group,Whitehead Institute for Biomedical Research)A genoe-wide gene expression analysis u
流式细胞仪技术专辑
最方便的实验干货查询工具微信扫码进入「丁香实验」小程序编辑： 呜咽分享到： Flow Cytometry Analysis (Springer Lab, Harvard University)Flow cytometry employs instrumentation that scan
Laser－Capture－Microdissection－(LCM)
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
Global－urinary－metabolic－profiling－procedures－using－gas－chromatography
Global urinary metabolic profiling procedures using gas chromatography–mass spectrometry Eric Chun Yong Chan,1 Kishore Kumar Pasikanti1, 2 & Jeremy K
CGH－Protocols－（四）
CGH Image acquisition Images were acquired through a Zeiss Axiophot fluorescence microscope using a Plan NEOFLUAR oil objective x63, N.A. 1.25 (Zeis
CGH－Protocols－（二）
DNA preparation by cryotom tissue dissection Preparations/Materials: Cool cryostat down to -20 to -30°C about 3 hours prior to dissection Label ep
CGH－Protocols－（一）
Metaphase chromosome preparation Materials: RPMI 1640 medium fetal calf serum (FCS), 20% Colcemid (e.g. Boehringer Mannheim cell biology reagen