DNA测序(主要内容如下)
· Sequencing Gel Preparation
· Preparation of Templates
· DNA Sequencing by the Dideoxy Method
· DNA Sequencing by the Chemical Method
· Dye Terminator DNA Sequencing
· Construction of Nested Deletions for Sequencing
· PCR Sequencing
· Recipes
· Q & A Posted in Molecular Biology Method Forum
· Sequencing Gel Prep (NWFSC)
Gel preparation, buffer, electrophoresis...
· Sequencing Gel Prep (NWFSC)
Wedge gel performance without pouring wedge gels
· Sequencing Gel Prep (Promega)
· Buffer Gradient Sequencing Gel (Bowtell Lab)
· Buffer Gradient Sequencing Gels (PMCI Research)
· Preparation of Template DNA for Sequencing (Promega)
· PCR Product Sequencing (Genetics Resources CORE Facility at Johns Hopkins U)
Detailed method for preparing PCR products for direct sequencing
· Plasmid DNA Preparation for Sequencing (Genetics Resources CORE Facility at Johns Hopkins U)
· Phage and Cosmid DNA Preparation for Sequencing (Genetics Resources CORE Facility at Johns Hopkins U)
· Double Stranded DNA Sequencing (Dideoxy) Double Stranded DNA Sequencing (Dideoxy) ( (Bowtell Lab)
Sequencing of Double Stranded DNA Using Dideoxy Chain Termination (Donis Keller Lab)
This method is used to sequence double stranded DNA, such as a plasmid insert or purified PCR product. The dideoxy chain termination (or enzymatic) method of DNA sequencing involves the in vitro synthesis of a DNA strand by a modified bacteriophage T7 DNA polymerase (SequenaseR/USB) using a single stranded DNA template.
· DNA Sequencing Reaction (Lazo Lab)
· Old Fashioned Sequencing(Brent Graveley)
· DNA Sequencing Using an End-Labeled Primer (Promega)
· DNA Sequencing Using Direct Incorporation (Promega)
· Double Stranded DNA Sequencing (Dideoxy) (PMCI Research)
DNA sequencing (Roe Lab)
i. Bst-catalyzed radiolabeled DNA sequencing
ii. Radiolabeled sequencing gel preparation, loading, and electrophoresis
iii. Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers
iv. Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions
v. Sequenase[TM] catalyzed sequencing with dye-labeled terminators
viii. cDNA sequencing based on PCR and random shotgun cloning
· Preparation of G+A Marker
Simplified method for preparing G+A ladder run along with footprinting reaction. It's much simple than the original Maxima-Gilbert sequencing reaction and works fine.
Dye Termination Sequencing of dsDNA. (Gimila Lab)
· ABI Dye Primer Reactions using FS (Whitehead Institute/MIT)
· ABI Dye Primer Reactions using ThermoSequenase(Whitehead Institute/MIT)
· ABI Dye Primer Reactions Home Brew(Whitehead Institute/MIT)
· ABI T7 Terminator Reactions(Whitehead Institute/MIT)
· ABI AmpliTaq Dye Terminator Reactions(Whitehead Institute/MIT)
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