Ruedi小组用QTRAP开发出全球首个定量蛋白质组数据库

苏黎世理工学院借助AB SCIEX的QTRAP技术研发成功全球首个蛋白质组学MRM Atlas

基于质谱的在线数据库加速蛋白质分析

　　福斯特市，加利福尼亚—2010年10月4日，生命科学分析技术的全球领导者AB SCIEX公司，今天宣布瑞士联邦理工学院(苏黎世理工学院，ETH)与西雅图的华盛顿大学系统生物研究所合作，使用新一代QTRAP质谱技术测定了近20,000个人体蛋白质，首创全球第一个人类多反应监测(MRM) Atlas数据库，MRMAtlas是一个能够实现数据共享的在线数据库。全球的科学家都可以使用atlas数据库，来显著提高生物标记物研究、基于蛋白的药物发现和基础生物及生物医学研究的效率。MRM Atlas提供了简单、认证的方法，可以将蛋白质组学研究拓展到前所未有的领域。该项目由Ruedi Aebersold 博士领导的ETH科学家完成，Ruedi 博士将出席11月9日播出的一个技术介绍类的现场网播节目，向大家介绍MRMAtlas。

Prof. Ruedi Aebersold

　　MRMAtlas是一个全新的数据库，提供了大量基于质谱的人类蛋白质组信息，使得研究者能够尽可能缩短冗长并且耗时的开发时间。许多科学家都将MRM视为现有免疫测试的改进方法，现有方法包括蛋白质印迹法(Western Blotting)和酶联免疫吸附测定(ELISA)等，这两种方法均使用抗体。用免疫测试是一种非常昂贵且耗时的开发方法，还有一系列特异性的问题。全新的MRM数据库与大型现成的特定蛋白质抗体数据库相似;区别在于，成百上千的蛋白质和多肽可用简单的质谱方法检测和定量，而不需要交叉反应。

　　MRMAtlas数据库的开发依赖于QTRAP技术，Qtrap独有的技术可在一次实验中结合两种能力——三重四极杆系统的定量MRM能力;先进的线性离子阱的蛋白质鉴定及确证能力。这给研究者提供了每个样本的清晰图像，减小了不确定性，满足了苏黎世理工学院科学家的要求。AB SCIEX QTRAP系统业界领先的灵敏度同样意味着，科学家可以开发出蛋白质组中低丰度蛋白测定的方法。使用QTRAP分析时，苏黎世理工学院的科学家开发了这一全面的MRM数据库，并可获得100个多肽/小时的空前速度分析合成多肽。

　　Ruedi Aebersold博士，教授，瑞士联邦理工学院生物技术研究所

　　“MRM方法将改变全世界的蛋白质组学研究。MRMAtlas是一种先进的蛋白质分析工具，这项由具有现身精神的科学家团队完成的工作是一个重要的里程碑，将把我们对人类蛋白质组分析的理解提高到一个新的层次。如果没有QTRAP技术，我们是不可能这么快速而高效地开发出MRMAtlas的。”

　　Andy Boorn，首席运营官，AB SCIEX

　　“AB SCIEX QTRAP系统帮助我们创建了MRMAtlas，而MRMAtlas也将会展示出质谱在蛋白质组分析方面的真正能力。科学家们从现在起将能够同时分析人体内数量巨大的蛋白质，这对于生物标记物研究和最终的个性化治疗将产生重大影响。QTRAP系统本质上是最好的三重四极杆，同时具有附加的和独特的能力。正如里程碑式的MRMAtlas项目一样，QTRAP将继续帮助科学家们取得重要的科学成果。”

　　关于AB SCIEX

　　AB SCIEX是生命科学分析技术开发领域的全球领先企业，帮助科学家应对复杂的科学挑战。公司提供科学仪器、软件和服务，用于发现新药、促进医学科学发展、保护食品供应链和环境。AB SCIEX的技术解决方案结合了最高的性能和最高的稳定性，确保我们的用户进行科学发现、可靠地传递结果和提高生命质量。AB SCIEX拥有近30年的创新历史，在市场中一直出于领先地位，其前身为美国应用生物系统公司(Applied Biosystems)和美迪希(MDS)实验仪器公司的合资企业。2010年1月30 日，AB SCIEX作为丹纳赫集团的子公司开始运营。更多资料请访问官网：www.absciex.com。

　　http://www.mrmatlas.org/

　　QTRAP技术概览

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　　QTRAP5500 四极杆-线性阱杂交质谱仪

ETH Zurich Develops the World's First Proteomics MRM Atlas with QTRAP Technology from AB SCIEX

Online database of mass spectrometry-based assays to accelerate protein analysis

　　FOSTER CITY, Calif. – October 4, 2010 – AB SCIEX, a global leader in life science analytical technologies, today announced that scientists at the Swiss Federal Institute of Technology (ETH Zurich), in collaboration with the Institute for Systems Biology in Seattle, WA, used next-generation QTRAP mass spectrometry technology to create assays for approximately 20,000 human proteins, generating the world’s first human Multiple Reaction Monitoring (MRM) Atlas, an online database that will be made publicly available. Scientists around the world can use this atlas to significantly advance biomarker research, protein-based drug development and fundamental biological and biomedical research. The MRM Atlas provides simple, validated assays expanding into never-before-seen areas of the proteome. This project involved ETH scientists led by Dr. Ruedi Aebersold, Ph.D., who will be featured in a November 9th live webcast of a technical presentation about the MRMAtlas.

　　The MRMAtlas is a new database that provides mass spectrometry-based assays to a large proportion of the human proteome, enabling researchers to minimize the tedious and time-consuming task of assay development. Many scientists see MRM assays as an improvement over current immunoassays, such as Western Blotting and ELISA, which use antibodies. Immunoassays can be very costly and time-consuming to develop and can have issues with specificity. The new database of MRM assays is similar to a large library of ready-to-go antibodies to specific proteins, but the difference is that hundreds of proteins and peptides can be detected and quantified in a simple mass spectrometry assay and without the issues of cross reactivity.

　　The development of the MRMAtlas was dependent on QTRAP technology, which uniquely combines in a single analysis the quantitative MRM capabilities of normal triple quadrupole systems, with advanced linear ion trap capabilities for protein identification and confirmation. This gives researchers a clearer picture of each assay and eliminates ambiguity, meeting the requirements of the scientists at ETH Zurich. The industry-leading sensitivity of the AB SCIEX QTRAP systems also means the scientists were able to develop assays to low abundant proteins in the proteome. Using synthetic peptides for the QTRAP technology to analyze, the scientists at ETH developed this comprehensive library of MRM assays with unprecedented speed of 100 peptides per hour.

　　Ruedi Aebersold, Ph.D., Professor, Institute of Biotechnology at the Swiss Federal Institute of Technology

　　“MRM assays are set to change the world of proteomics. The MRMAtlas is a tool for advancing protein analysis. This accomplishment by a dedicated group of scientists is an important milestone and will improve our understanding of the analysis of the human proteome. We could not have developed the MRMAtlas as quickly and productively without QTRAP technology.”

　　Andy Boorn, Chief Operating Officer, AB SCIEX

　　“The adoption of AB SCIEX QTRAP systems to help create the MRMAtlas will unlock the real power of mass spectrometry for proteomics analysis. Scientists will now be able to analyze huge numbers of proteins in the human body all at the same time, which has significant ramifications for biomarker research and ultimately personalized medicine. The QTRAP systems are essentially the best triple quadrupoles available but with additional, unique capabilities. They continue to help deliver significant scientific results, such as this landmark MRMAtlas project.”

　　About AB SCIEX

　　AB SCIEX is a global leader in the development of life science analytical technologies that help answer complex scientific challenges. The company provides scientific instrumentation, software and services used to discover new drugs, advance medical science and protect the food supply and the environment. AB SCIEX technology solutions combine the highest performance with the highest reliability to enable our customers to fuel scientific discovery, deliver results with confidence and improve the quality of life. The company has a more than 20-year history of innovation and market leadership as the former Applied Biosystems/MDS Analytical Technologies joint venture. For more information about AB SCIEX, go to www.absciex.com. Follow AB SCIEX on Twitter @ABSCIEX and on Facebook.