WIKI资讯
WIKI资讯
Flow Cytometric AnalysisPrior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspensions to a final concentration of 5 μg/mL and incubated for 10 min at 37°C in the dark. Cells were analyzed by means of a MoFlo Cytometer (DakoCytomation) equipped with a UV excitation wavelength laser (Innova 90C-6) operating at 25 mW and a 70-μm nozzle.Ana......阅读全文
C. Random fragment end-repair, size selection, and phosphorylationSince both sonicated and nebulized DNA fragments usually contain single-stranded end
Lysate preparation and western blottingProtein lysates were created by harvesting the cells from confluent T-flasks or from suspension cultures at h
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Cor
Picking ES cell clonesOne or two days before picking colonies prepare 24-well plates of feeders. You can also use alternate protocols that utilize 96-
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zúñiga-Pfl
A Method for Micro-Scale Isolation and Purification of GangliosidesStephan Ladisch~Director, Center for Cancer and Transplant Biology, Children's
Cytokine BioassaysIntroductionBiological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary cel
实验概要The CellTrace™ CFSE Cell Proliferation Kit provides a versatile and well-retained cell-tracing reagent in a convenient and easy-to-use
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
Growth and storage of Agrobacterium tumefaciensStrain GV3101: resistant to gentamycin and rifampicin so add 25-50 ug/ml Gentamycin, 10 ug/ ml rifampic
Description Transform E.coli cells with plasmid/cosmid DNA using the method of electrophoration (inserting plasmids into E.coli).A
三、ICC在细胞功能研究中的应用 形态学研究目的之一是揭示组织细胞结构特征及其功能意义。利用ICC显示给与细胞增殖标记物，是组织细胞学研究中形态和功能相结合的重要手段之一。目前常用的细胞增殖标记物有4种，Brdu (5—bromo—2--deoxyuridine), DNA polymerase
General Issues and RequirementsSurgery is defined as any procedure that exposes tissues normally covered by skin or mucosa. Experimental surgery
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
3. Commentary 3.1. Background informationApoptosis is an innate mechanism of eukariotic cell suicide which plays a major role i
Purification of plasmid DNA (miniprep) with high yields using diatomaceous earthKyung-Soo Kim and Charles K. PallaghySchool of Botany, La Trobe Univer
DNA PreparationE. coli chromosomal DNA is prepared following the method of Heath et al. ( J. Bacteriol., 174, 1992). Cells are embedded in agarose, th
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excit
Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a gi
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
B.3. COMMENTARY B.3.1 Background information The critical steps in the methodology are cell fixation, permeabilization and the concentration
Intestinal intraepithelial lymphocytes Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously
实验概要Adenoviral vectors widely used to transfer foreign genes into neuronal cells possess tropism for glial cells and are toxic to infected cells.
The Transmission Electron Microscope (TEM) (HEI)An explanation of how the TEM works. TEM Specimen Preparation (HEI) Se
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
实验概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
实验概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apopto
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
Preparation Of Ciliated Protozoa For Scanning Electron MicroscopyGeneral notes: The same procedures are used to fix and stain cells for SEM and for TE
1. Preparation of coverslips1.1- Mass cultureOur standard mass cultures are plated on astrocytes. Those, in turn, are plated on glass cover