SFX研究有哪些实例和参考文献可供参考？

Figure 9 PS-I低分辨X－ray nanocrystallography 结构解析装置示意图及结构

答：第一次实验。2011年，Henry Chapman在美国建成的LCLS自由电子激光装置上成功获得了光合反应中心I（PS-I）晶体的低分辨衍射数据。他们将尺寸在0.2-2微米的PSI晶体输送到自由电子激光光束中，获得了衍射数据。这些低分辨的衍射数据和在同步辐射上获得的同样晶体的数据完全一致，证明了利用XFEL可以获得准确的晶体衍射数据。

Reference: Chapman, H.N., et al., Nature, 470 7332 (2011).

Figure 10 溶菌酶晶体的高分辨衍射数据

第一次室温下高分辨。2012年Henry Chapman研究组利用LCLS的XFEL获得了溶菌酶晶体的高分辨衍射数据，并利用分子置换法解析出了晶体结构。这次实验使用的晶体尺寸很均一，为1X1X3μm3，获得了1.8埃分辨率的溶菌酶晶体在常温下的结构。此结果表明XFEL可以获得高分辨的衍射数据，而且能够解析出晶体结构。

Reference: Boutet, Sebastien., et al., Science, 337 6092 (2012).

Figure 11 一种GPCR的XFEL 结构解析

第一次GPCR等膜蛋白实验。Henry Chapman报道了利用XFEL得到的GPCR的高分辨结构该实验证明了膜蛋白结构也可以在XFEL上获得，对于结构生物学研究者是很有吸引力的。

Reference: Liu, et al., Science, 342 6165(2013).

此外，还有大量的化学反应下的研究，动态构象变化的研究，泵浦下结构与性质的变化研究。代表性参考文献：

1. H.N. Chapman, et al.,  "Femtosecond X-ray Protein Nanocrystallography" Nature 470, 73–77 (2011) .

2. Sébastien Boutet, et al., "High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography" Science 337 362 (2012) .

3. Lars Redecke, et al., "Natively Inhibited Trypanosoma brucei Cathepsin B Structure Determined by Using an X-ray Laser" Science 338 (2012).

4. Jan Kern,et al., "Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature" Science (2013).

5. D. Milathianaki, et al., "Femtosecond Visualization of Lattice Dynamics in Shock-Compressed Matter" Science 342 6155 (2013) .

6. Thomas R. M. Barends, et al., "De novo protein crystal structure determination from X-ray free-electron laser data" Nature -- (2013) doi: 10.1038/nature12773.

7. Wei Liu, et al.,  "Serial Femtosecond Crystallography of G Protein–Coupled Receptors" Science 342 1521 (2013).

8. Christopher Kupitz, et al., "Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser" Nature --- (2014) doi: 10.1038/nature13453.

9. Jason Tenboer, et al.,  "Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein" Science 346 1242 (2014) .

10. Yanyong Kang, et al.,  "Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser" Nature --- (2015) doi: 10.1038/nature14656.

11. Jose A. Rodriguez, et al., "Structure of the toxic core of α-synuclein from invisible crystals" Nature -- (2015) doi: 10.1038/nature15368.

12. Jose A. Rodriguez, et al., "Structure of the toxic core of α-synuclein from invisible crystals" Nature -- (2015) doi: 10.1038/nature15368.

13. Kanupriya Pande, et al., "Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein" Science 352 725(2016) .

14. J. R. Stagno, et al.,  "Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography" Nature --- (2016) doi: 10.1038/nature20599.

15. Iris D. Young, et al.,  "Structure of photosystem II and substrate binding at room temperature" Nature --- (2016) doi: 10.1038/nature20161.

16. A. Rudenko, et al., "Femtosecond response of polyatomic molecules to ultra-intense hard X-rays" Nature 546 129 (2017) doi: 10.1038/nature22373.

只列举了我认为在不同方面具有突破性进展及影响力的Nature 及Science 文章。