单克隆抗体

·         Tips and hints for the storage of antibodies (Synaptic Systems)

·         Purification of IgG Using Protein A- or Protein G-Agarose(KPL)

·         Production  of Monoclonal Antibody (Chisholm Lab)
Protocol for making monoclonal antibodies. All steps of the entail procedure are described in great details

·         Production of Monoclonal Antibody in Mice  (Walter Steffen)
Whole procedures for monoclonal antibody production

·         Preparation of Monoclonal Antibody (PMCI)
Protocol for making hybridoma and antibody

• Cell Fusion and Selection (PMCI)

• Fusion of Mouse, Rat, or Hamster Cell (Contributed by Nanci Donacki)
    

• Fusion and Cloning (Contributed by Nanci Donacki)
  Procedure for establishing hybridoma in one step
    

• Cloning by Limiting Dilution (Contributed by Nanci Donacki)
    

·         Affinity Purification of Monoclonal Antibodies From Tissue (PMCI)
  

·         Expanding Hybridoma Clones (PMCI)
   

·         Protein G Purification of Antibody(Contributed by Nanci Donacki)
  

·         Protein A Purification of Antibody (Contributed by Nanci Donacki)