· Histological techniques (William H. Heidcamp)
Very detailed guide to histological techniques, like fixation, dehydration, embedment and subsequent sectioning.
· 3-Aminopropyltriethoxysilane Treatment of Slides(Nottingham Pathology Lab)
· Paraffin-Embedded Tissue for LCM (Arcturus)
Paraffin embedding is a process in which fixed (formalin or other fixative) is infiltrated and then blocked out in paraffin to stabilize it for long-term storage and easy sectioning...
· Wax embedding/sectioning (Klymkowsky Lab)
Chrome Gelatinised Slides and Coverslips (Nottingham Pathology Lab)
Decalcification (Nottingham Pathology Lab)
Many surgical specimens contain calcified areas which need to be decalcified before processing and sectioning. This is the protocol used for this purpose
De-Paraffinization of Tissue Sections (Arcturus)
Remove paraffin from section for tissue preparation such as nucleic acid extraction.
DNA Extraction From Paraffin Section (Arcturus)
This extraction method has been used for measuring loss of heterozygosity (LOH), dideoxy fingerprinting (DDF), clonality analysis (chromosome X inactivation) and direct sequencing of PCR* products for single base mutational analysis. Extractions are typically performed on 500-1000 captured cells.
RNA Extraction from Paraffin Section (NIH Protocol) (Arcturus)
A Protocol for extracting RNA from paraffin embedded tissue.
Preparation of Frozen Tissue for LCM (Arcturus)
White Blood Cell Cytospin (Arcturus)
