Bruker推出新一代ultrafleXtremeMALDITOF／TOF质谱仪

　　2009年4月27日，布鲁克道尔顿公司推出新一代ultrafleXtreme MALDI-TOF/TOF质谱仪，这是市场上唯一的，在TOF和TOF/TOF模式下都能获得真正的1 kHz采样速度的仪器，可用于广泛的研究、临床、和应用蛋白质组学。

　　布鲁克的新型ultrafleXtreme质谱仪，具有1 kHz smartbeam-II激光束技术，可应用于蛋白质组学、生物标志物发现和定量、生物医药的质控、组织分子成像和从上到下(Top-Down)的测序。

　　在蛋白质组学研究中，MALDI-TOF/TOF技术是ESI-MS/MS技术强有力的补充手段，这类ESI-MS/MS技术包括：布鲁克带ETD解离技术、高容量的离子阱，和超高分辨的maXis (UHR) ESI-Q-q-TOF系统，在现代的蛋白质分析中，MALDI和ESI技术都需采用，以获得最高的序列覆盖度和灵活性。

　　新型ultrafleXtreme合并了几大发明：布鲁克独一无二的1 kHz smartbeam-II激光技术，最新的离子光学技术。它是唯一的获得1 kHz高通量的MALDI-TOF/TOF，可用于生物标志物发现和定量;同时，激光束的尺寸非常接近于单细胞的空间分辨，可高效地进行组织分子成像。 LC-MALDI技术也能获得无与伦比的高分辨率、精确质量和高速的数据采集。Bruker独一无二的Edmass 蛋白测序能力，可对天然蛋白进行快速和灵敏的蛋白表征，可以测得高至80个残基的序列，包括直接分析N-端和C-端修饰。

　　ultrafleXtreme集成了业界领先的TOF/TOF技术，包括：

　　新型的1 kHz smartbeam-II激光，对于MS和MS/MS都可以进行快速的数据采集。可进行蛋白组织成像、天然蛋白分析、生物或寡核苷酸的质控，LC-MALDI的蛋白质组分析——所有都可在1 ~ 1000 Hz调制。

　　宽带质量分辨率高达40,000，可进行精准的蛋白质组学分析，经由Bruker独特的PAN技术，可获得跨越宽阔质量范围的高分辨率，而不仅仅是针对某一小段质量数。

　　新型的FlashDetector检测器合并了4 GHz的数字转换器，可获得40,000分辨率和 1 ppm质量准确度，可使结果更为确信。

　　Bruker专利的smartbeam技术，已被公认为是激光技术中最多样化的MALDI成像技术。ultrafleXtreme如今可获得小至10 µm直径的激光聚焦，获得高的空间分辨率，没有像素重合。重要的是，在如此小的激光束直径下，还可以获得出众的谱图质量和信号强度。

　　新型独特的激光-红外自清洗MALDI源，可获得长时间稳定的高性能。非常长的MALDI激光束寿命，加上自动的源清洗功能，可获得最高的运行时间，降低了维护的成本。

　　Bruker全面的软件平台上，集成了各种有效的数据处理方式：

　　ProteinScape 2.0确保获得定量研究的确定性高的信息：不管是从Label-free(非标记)的方法，或是ICPL-4 plex，SILAC，iTRAQ-8plex，还是其它用户自定义的标记技术。它集成了大规模蛋白质组学研究的多种数据组，并根据标准的、公开的原则产生报告。

　　BioTools 3.2是市场上领先的生物信息学平台，现在可对重组蛋白进行top-down蛋白测序，比如对于生物治疗和生物相似物的表征和质量控制。

　　flexImaging 2.1是基于MALDI 组织分子成像平台上的生物标志物发现的黄金标准，全面兼容于临床组织学。

　　布鲁克.道尔顿公司的执行副总裁 Ian Sanders博士评论说：“ ultrafleXtreme具有当今MALDI-TOF/TOF质谱无与伦比的灵活性和性能。研究者们可以利用它在蛋白质组学和MALDI成像方面获得更新的进步，即使在很短时间以前，研究者们还仅是梦想能在如此宽的范围内获得这样的数据质量。”

　　ultrafleXtreme的性能将要在5月31日，2009年第57届ASMS上在Bruker的用户会上展示。

　　Bruker Daltonics today launches its next-generation ultrafleXtreme MALDI-TOF/TOF mass spectrometer, the only instrument combining true 1 kHz speed in both TOF and TOF/TOF modes with ultra-high performance and extreme flexibility for a broad variety of complementary research, clinical and applied proteomics applications.

　　Bruker's Novel ultrafleXtreme(TM) with 1 kHz smartbeam-II(TM) Laser Technology Redefines MALDI-TOF/TOF Performance for Proteomics, Biomarker Discovery and Quantitation, Biopharma QC, Tissue Molecular Imaging and Top-Down Sequencing (Photo: Business Wire)

　　In proteomics, MALDI-TOF/TOF is highly complementary to ESI-MS/MS with Bruker's latest high-capacity ion traps with ETD, and to ultra-high resolution (UHR) ESI-Q-q-TOF maXis systems, as both MALDI and ESI are required for highest coverage and flexibility in modern protein analysis.

　　The new ultrafleXtreme incorporates key inventions, Bruker's proprietary smartbeam-II laser technology at 1 kHz, and the latest ion optics innovations. It is the only MALDI-TOF/TOF instrument that truly enables high-throughput 1 kHz biomarker discovery and quantitation, as well as fast, high-performance tissue molecular imaging with a laser spot size close to single cell spatial resolution. LC-MALDI can be performed with unmatched mass resolution, mass accuracy and data acquisition speed. Bruker's unique Edmass protein sequencing capabilities allow very rapid and sensitive intact protein characterization and sequencing with read lengths up to and beyond 80 residues, and with direct analysis of N- and C- terminal modifications.

　　Industry-leading TOF/TOF technology is incorporated into the ultrafleXtreme for unprecedented capabilities:

　　The novel full 1 kHz smartbeam-II laser enables ultra-high data acquisition speed in both MS and MS/MS at full systems performance. The well-established proprietary smartbeam laser provides unprecedented analytical and matrix flexibility in workflows from protein tissue imaging, intact proteins analysis, biologics or oligo QC, to LC-MALDI proteomics - all fully enabled at 1-1000 Hz repetition rates.

　　Broadband mass resolving power up to 40,000 enables precision proteomics via Bruker's unique PAN technology for highest mass resolution across a very broad mass range, not just at a selected optimum.

　　The novel FlashDetector TM combined with a new 4 GHz digitizer and latest advances in electronics provide unmatched mass resolving power up to 40,000 and 1 ppm mass accuracy for highest confidence.

　　Bruker's patented smartbeam technology is already widely accepted as the most viable MALDI imaging laser technology. The ultrafleXtreme now enables laser focus diameters down to 10 µm for high spatial resolution imaging without pixel overlap. Importantly, outstanding spectral quality and signal intensity are maintained at even the smallest laser beam diameters.

　　The novel and unique laser-irradiation self-cleaning MALDI Perpetual I on S ource ensures robust, long-term highest-performance operation. Very long MALDI laser lifetime in combination with automated source cleaning in just minutes leads to high uptime and low maintenance costs.

　　Efficient data retrieval and interpretation is provided by fully integrated software platforms designed, progressed and supported by Bruker with leading proteomics experts, for:

　　ProteinScape 2.0 ensures confident information from quantitative studies using either label-free or ICPL - 4plex, SILAC, iTRAQ-8plex or user-defined label technologies, integrates multiple datasets across large proteomic studies and generates reports in accordance to standard publication guidelines.

　　BioTools 3.2 is the market-leading bioinformatics platform, now extended to top-down protein sequencing for recombinant protein work, e.g. in biotherapeutics and biosimilars characterization and QC.

　　flexImaging 2.1 is the true gold standard in biomarker discovery based on MALDI tissue molecular imaging and is fully compatible with clinical histology .

　　Bruker Daltonics Executive Vice President Dr. Ian Sanders commented: "Its incredible flexibility and unparalleled performance make the truly innovative ultrafleXtreme the definitive MALDI-TOF/TOF today. Researchers are enabled for new achievements in proteomics and MALDI imaging, while achieving result quality across the board that would have been undreamed of even a short time ago."

　　The capabilities of the new ultrafleXtreme will be highlighted at Bruker's Annual Users Meeting on Sunday, May 31 st , 2009 before the 57 th ASMS Conference on Mass Spectrometry in Philadelphia, PA. For more information on this event, please visit www.bdal.com/asms2009 . For further product information please visit www.bdal.com/ultrafleXtreme .

　　ultrafleXtreme, ProteinScape, PAN, smartbeam , FlashDetector, MALDI Perpetual, BioTools, ProteinScape, FlexImaging are trademarks of Bruker Daltonik GmbH. ICPL is a trademark of TopLab GmbH, and iTRAQ is a trademark of Applied Biosystems.