发育生物学

In Vitro Production of Bovine Embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
This protocol describe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)]of embryos

Mini-Chamber for regulating gaseous environment during culture(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposable media-filtration devises such as Corning’s 115-ml system.  The following is a description of how to make such a device

Cell number counting in preimplantation embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A simple procedure for determining cell number in preimplantation embryos. While the procedure is written for fixed embryos, fixation is optional since the Hoechst 33342 and 4',6-diamidino-2-phenylindole, dihydrochloride (DAPI) penetrate living cells. The fixation step is useful when embryos will be stored for several weeks before determination of cell number

TUNEL procedure for preimplantation embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A protocol for detecting cell death using TUNEL.

Whole mount TUNEL analysis of Xenopus embryos (Peter Vize, Xenopus Molecular Marker Resource)

Lineage Analysis Using Retrovirus Vectors (Cepko Lab, Department of Genetics, Harusr/locald Medical School and Howard Hughes Medical Institute,)
Describe the use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues.