Making Antibody
· Production of Polyclonal Antibody in Rabbit (Walter Steffen)
Provides detailed protocol for immunizatioin, bleeding procedure, obtaining serum, precipitation of IgG.
· GST-fusion protein injection into rabbits (IVAN J. DELGADO ORLIC)
· How Do I make an Antibody against a Peptides (Pierce)
· A fast and inexpensive method for raising high-specificity polyclonal antibodies in mice (Technical Tips Online)
· Guide to Antipeptide Antibody (Perkin-Elmer)
General Information about the design and synthesis of peptides for the production of anti-peptide antibodies, as well as an overview of the steps involved in the production of antibodies immunization, collection of antisera, analysis of antisera, and antibody purification. Protocols for a number of these procedures are provided.
o Conjugation of Peptides to Protein Carriers
o Analysis of Antisera: Immunoassays
Preparation of Polyclonal Antibodies (PMCI)
Immunization and antisera preparation
Making antibodies to synthetic peptides (Daniel T. O'Connor)
Preparation of Peptide-KLH Conjugates for Immunization(Mitshison Lab)
Testing Rabbit Bleeds by Elisa (Mitshison Lab)
Coupling Peptides to Resin for Affinity Purification (Mitshison Lab)
Purification of Anti Peptide Antibody (Mitshison Lab)
Others
· Tips and hints for the storage of antibodies (Synaptic Systems)