Biotinylation of Antibody ( Contributed by Nanci Donacki)
Antibody labeling (House Ear Institute)
Labeling protocols using affinity markers.
Coupling Colloidal Gold to Protein (Walter Steffen)
Gold labelling of rabbit IgG
Preparation of enzyme gold complex
Coupling of Nanogold to IgG
Production of Colloidal Gold (Walter Steffen)
Protein (Antibody) Fluorescent Labeling (Mario Roederer)
Detailed protocol for conjugation of proteins (principally monoclonal antibodies) to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V). Procedures for the conjugation of immunoglobulins are provided for the following molecules:
In addition, protocols for conjugation of Annexin V to the following molecules are given as models for fluorescent conjugation of non-immunoglobulin proteins.