用Bravo和AssayMAP高通量纯化人IgG蛋白

High throughput purification of human IgG using the Agilent Bravo for Protein Purification and AssayMAP protein A cartridges

Abstract:

To address a growing desire for robust, high-throughput chromatography capability, Agilent Technologies has developed a 96-channel liquid handling head to enable microchromatography on the Bravo Automated Liquid Handling Platform. using AssayMAP protein purification cartridges from BioSystem Development. This system is called the Agilent Bravo for Protein Purification. The system can operate using direct fluid displacement for chromatography applications, or air displacement for conventional liquid handling. Early results using the system to purify immunoglobulin from artificially constructed samples show the system can purify between 1 and 100 μg of target protein when presented in backgrounds as high as 10 mg/mL of non-specific protein. Recovered protein quantities accurately reflect input quantities, and these results are repeatable over a number of purifications using used or unused cartridges.

Keywords: Bravo, AssayMAP, HT, high-throughput, purification, chromatography, microchromatography, biologics, pharmaceutical, biopharmaceutical, VWorks