组织学一般技术

The manuals for this section have been compiled by Susan L. Wall, HT(ASCP). These manuals are in Adobe Acrobat PDF format and require the Acrobat Reader for viewing.

Staining Manuals

• Fixative Solutions: Each of the following .PDF files contains the procedure plus a sample container label.

• 95% Alcoholic Bouin's Fixative

• 95% Alcohol Fixative

• B5 Fixative

• Bouin's Fixative

• Formalin Fixative

• Karnovsky's Fixative (glutaraldehyde)

• Hartman's Fixative

• Hollande's Fixative

• Orth's Solution (dichromate fixative)

• Zenker's Fixative

• Stock Solutions: Each of the following .PDF files contains the information regarding the use and preparation of a working solution.

• Acid cleaning solution for glassware

• Bluing solution for hematoxylin stain

• Celloidin slide adhesive

• Chrome alum adhesive

• Glycerin water mounting medium

• H and E acid decolorizing rinse

• Hypo (sodium thiosulfate)

• Lugol's iodine for mercury removal

• Metal embedding mold release

• Paraffin block softening solution

  
  

• Miscellaneous Labels

• Staining Procedures: Each of the following .PDF files contains the procedure for a special stain, a procedure card, and sample container labels for the reagents.

• Introduction

  
  

• Acid Fast Stain (for mycobacteria)

• Acid Fast Stain (Ziehl-Neelsen, for M. leprae and Nocardia)[contributed by Greg Winn, Specialist Histopathology & Cytology Services, Western Australia]

• Fite's Acid Fast Stain

• Alcian Blue Stain

• Alcian Blue-PAS Stain (PAB)

• Hyaluronidase Digestion for Alcian Blue

• Alizarin Stain for calcium

• Auramine-Rhodamine Stain (fluorescent)

• Bielschowsky Stain (for senile plaques)

• Bile Stain

• Bodian's Stain

• Colloidal Iron Stain

• Congo Red Stain

• Copper Stain

• Elastic van Gieson Stain

• Elastic - Weigert's resorcin-fuchsin method

• Modified Elastic van Gieson Stain

• Fontana-Masson Stain for melanin

• Melanin Bleach

• Fraser Lendrum Stain

• Giemsa (Modified May-Gruenwald) Stain (for hematopoietic tissues)

• Giemsa Stain (for Helicobacter)

• Gram (Modified Brown-Brenn) Stain

• Gridley's Stain for ameba

• Grimelius Argyrophil Stain (Pascual's Method)

• Grocott's Methenamine Silver (GMS) Stain

• Holzer's Glial Fiber Stain

• Hortega's Pineal Stain

• Iron Stain (Prussian blue)

• Iron Stain (Turnbull's blue)

• Jones' Silver Stain

• Luxol Fast Blue (LFB) Stain

• Methyl Green Pyronin (MGP) Stain

• Mucicarmine Stain

• Nissl Stain

• Oil Red O Stain

• Orcein Stain

• Periodic acid-Schiff Stain (PAS)

• Periodic acid-Schiff, digested Stain (PAS-D)

• PTAH Stain

• Reticulin Stain

• Spirochete Stain (Steiner & Steiner method)

• Sudan Black B Stain (for lipochrome)

• Sudan Black B Stain (for fat)

• Trichrome Stain - Masson's method

• Trichrome Stain - microwave method

• Thioflavin S Stain (for amyloid in tissues)

• Modified Thioflavin S Stain (for senile plaques)

• Toluidine Blue Stain (for mast cells)

• Urate Crystal Stain

• VonKossa Stain for calcium

Safety Procedures

• Bloodborne Pathogens (Exposure Control Plan)

• Working with Chemicals

• Chemical Hygiene Safety

• Chemical Inventory

• Emergency Procedures

• Facilities (engineering controls)

• Safety Equipment (engineering controls)

• Training Records

• Tuberculosis Guidelines