Tyrosine Kinase Assay Using Synthetic Peptides (T. Miller)
Small synthetic peptide substrates are especially well suited for applications such as assays of tyrosine kinases in permeabilized cells or for enzyme kinetic studies. This is a detailed protocol for tyrosine kinase assay.
Recombinant PKA Catalytic Subunit Preparation (S. Taylor)
Recombinant His-6 PKA Catalytic Subunit Purification (S. Taylor)
Recombinant His-6 PKA Regulatory Subunit Purification (S. Taylor)
Imunoprecipitation / In Vitro Kinase Assay (Valera Vasioukhin)
Detailed protocol for phosphorylation analysis, including cell lysate preparation, immunoprecipitation, in vitro kinase assay, autophosphorylation, and trouble shooting...
Immunopurification of Tyrosine Phosphorylated Proteins (Transduction Laboratories)
Protein Kinase Assay with an Immunocomplex (Translab)
SAPK/Jun Kinase Assays (Woodgett Lab)
Detailed protocol with recipes
Non-TCA Phosphoamino Acid Determinations (Woodgett Lab)
Phosphoamino Acid Analysis (Sefton Lab)