Day -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.
______________
Day 1: Trypsinized the cells as for normal passaging until the colonies lift off. Try to keep the loosely connected clumps of cells together by gentle handling. Then directly plate the cells 1:3 into bacterial grade Petri dishes in LIF free ES cell medium.
Day 3: Aspirate the medium carefully. Avoid sucking up too many of the aggregates. Then add new medium.
Day 5: Aspirate as in Day 3 and replace the medium. At this stage the differentiation the aggregates are called simple embryoid bodies.
Day 7: Change the medium
Day 9: Change the medium
Day 11: Change the medium
Day 13: Change the medium Day 15: By this day a high percentage of the embryoid bodies will have developed fluid filled cavities. The structures are called cystic embryoid bodies
分析测试百科网讯 近日，珀金埃尔默宣布收购总部位于美国新泽西州西柏林的ESIndustries公司，并将该公司最先进的色谱柱及化学试剂加入其液相色谱耗材组合产品线。ESIndustries以......
自然杀伤细胞（Naturalkillercells，NKcells）是一种效应性淋巴细胞，在人体抗肿瘤过程中发挥重要作用，但是，仍然有一些肿瘤细胞能够逃避机体NK细胞的攻击，逐步发生发展，侵袭转移，严......
研究人员发现了小鼠体内一种新的转录因子可以帮助调节间充质干细胞（mesenchymalstemcells，MSCs）分化为骨的过程。目前科学家们对于骨细胞分化的研究并不深入，而MSCs则是再生医学领域......
经过几十年的努力，科学家最终成功地从牛身上获得胚胎干（ES）细胞，并在培养皿中使其保持原始状态。获得这些可变成从皮肤到肌肉、骨头等各种组织的多功能细胞，将使调整和保存肉牛以及乳牛品种的遗传性状变得更加......