Protocols | |
 | |
| Downloadprotocol183kbpdf?Abstract for Gel Shift Assay SystemsThe gel shift, or electrophoretic mobility shift, assay provides a simple and rapid method for detecting DNA-binding proteins. This method has been used widely in the study of sequence-specific DNA-binding proteins such as transcription factors. The assay is based on the observation that complexes of protein and DNA migrate through a non-denaturing polyacrylamide gel more slowly than free DNA fragments or double-stranded oligonucleotides. The gel shift assay is performed by incubating a purified protein, or a complex mixture of proteins (such as nuclear or cell extract preparations), with a 32P end-labeled DNA fragment containing the putative protein binding site. The reaction products are then analyzed on a nondenaturing polyacrylamide gel. The specificity of the DNA-binding protein for the putative binding site is established by competition experiments using DNA fragments or oligonucleotides containing a binding site for the protein of interest or other unrelated DNA sequences.Promega has developed Gel Shift Assay Systems that contain target oligonucleotides, control extracts containing DNA-binding proteins, binding buffer and reagents for phosphorylating oligonucleotides. The Core System includes an E. coli extract containing cloned AP2 protein (AP2 Extract) and an AP2 Consensus Oligo. This is a reliable system for obtaining experience with gel shift assays because AP2 binding activity is stable and produces a strong gel shift. In addition, the Core System contains an SP1 Consensus Oligo, Gel Shift Binding 5X Buffer and sufficient HeLa Nuclear Extract to perform 20 control reactions. The complete system contains five additional double-stranded oligonucleotides that represent consensus sequences to characterized binding sites. These oligonucleotides can be end-labeled and used as protein-specific probes or as specific or nonspecific competitor DNA in competition assays. |
AMD宣布将以49亿美元的价格收购ZTSystems，这是一家专注于人工智能（AI）和云计算数据中心设备设计与制造的公司。这项收购是AMD历史上最大的一次收购之一，显示出该公司在AI领域要和Nvidi......
为迎合高速发展的半导体市场需求和逐渐增加的产品线，韩国ParkSystems于2023年9月15日在韩国果川市隆重举行了ParkSystems新总部大楼的奠基仪式。伴随着同期ParkSystems在韩......
2023年9月18日，韩国ParkSystems再次隆重入选“福布斯亚洲2023年十亿美元以下最佳企业”榜单。此次入选标志着ParkSystems继2020年跻身福布斯200强之后，第二次荣登著名的福......
一切从新，华丽启幕。2023年5月8日上午，ParkSystems上海实验室乔迁开幕式在上海德必虹桥国际WE盛大举行。上午十点，开幕仪式在在四起的掌声和绚烂的礼花中正式启动，来自原子力显微镜相关行业的......
将 AssayMAP 的使用范围扩展至法规监管环境2022年11月28日，北京——安捷伦科技有限公司（纽约证交所：A）近日宣布推出AssayMAPBravo蛋白质样品前处理Work......
分析测试百科网讯2015年10月6日，ParkSystems宣布其子公司ParkSystemsJapan与日本电子签订ParkSystems原子力显微镜产品在日本市场的分销协议。日本电子与ParkSy......
分享：HNMRshift......
艾美捷AssayGenie GeniePlex鼠标Th1/Th2/Th177-Plex(96次测试)：名称： 精灵小鼠 Th1/Th2/Th177 重套件编号：......
艾美捷AssayGenie重组人干扰素β：货号 KPRT0629类型 重组蛋白数量 5微克物种 人目标 IFN 测试版别名 IFN......
艾美捷AssayGenie 大鼠神经发现阵列（19个靶点）：型号： SARB0105大小： 2、4 或 8检测到的物种： 鼠检测到的目标数量......