| PROCEDURE |
| 1. Resuspend tissue/extract in 5 volumes of 1:1 chloroform/methanol by vortexing (to homogeneity or as close as possible) |
| 2. Add the same volume of a 1M KCl, 0.2M Phosphoric acid solution and vortex again |
| 3. Centrifuge sample at 10,000 xg for 2 minutes |
4. Collect the bottom phase (contains lipids) |
分析测试百科网讯2015年8月16日,中国仪器仪表学会分析仪器分会样品制备专业委员主办的第二届全国样品制备学术报告会在贵阳举行。本次大会与中国仪器仪表学会分析仪器分会2015学术年会同期举办,参会20......
1)TheyeastspeciesSaccharomycescerevisiaewereculturedinSDmediawith2%raffinose.Beforeextraction,yeastw......
1)TheyeastspeciesSaccharomycescerevisiaewereculturedinSDmediawith2%raffinose.Beforeextraction,yeastw......