Mouse Pulmonary surfatcant-associated protein A,SP-A ELISA Kit使用原理
小鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒
货号:FK-QZ3080
规格：96T/48T
Mouse Pulmonary surfatcant-associated protein A,SP-A ELISA Kit使用原理
Thekit
was a solid phase sandwich enzyme-linked immunosorbent assay(ELISA),
and the AVP concentrations of the standard samples and
unknownconcentrations of samples were detected by the addition of
microporousenzyme plates.. At the same time, the AVP and the biotin
labeledantibodies were incubated at the same time.. After washing, the
additionof Pro labeled HRP. After the warming and washing, the removal
of thenon binding enzyme conjugate, and then added to the substrate a,
B, andthe enzyme conjugate at the same time. Produce color. The color
depthand the concentration of AVP in the sample was proportional to
theconcentration of the sample..
Operation notes
1
reagent should be stored in the label manual, before using to
roomtemperature. The standard after dilute dilute should be discarded,
donot save.
2 the experimental plate should be immediately put back in the bag,seal the preservation, lest metamorphism.
3 no other reagents should be packaged or well. Do not mix differentbatches of reagents. Use before the shelf.
4
use a disposable suction head lest cross contamination, draw
thetermination liquid and substrate B, a liquid, to avoid using the
metalpart of the sample.
5 use clean plastic containers to configure the washing liquid. Allsamples of ingredients and mix well before use in the kit.
6
wash the enzyme plate should be fully dry, do not absorb water
paperdirectly into the enzyme labeled pores in the water absorption.
7
substrate a should be volatile, to avoid prolonged open lid.Substrate B
is sensitive to light, avoiding prolonged exposure to light.To avoid
contact with hand, toxic. After the experiment, read the ODvalue
immediately..
8 add reagents should be consistent in order, to ensure that allreaction plate hole incubation time.
9
to carry out the temperature fertility operation according to thetime,
the amount and the order of the liquid added in the instruction manual.
Kit performance:
1 the correlation coefficient r value of linear regression and theexpected concentration of sample was 0.92 above.
The 2 batch and the batch should be less than 9% and 15% respectively.
Detection range:
1.5 -90 g/ml g/ml
Save condition and its validity:
1 reagent kit preservation: 2-8.
2 validity: 6 months
小鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒