· RNA Gel (Crawford Lab)
· Gel Electrophoresis of RNA (Beverly Faulkner-Jones)
great tips on RNA gel electrophoresis.
· Northern Gel and Transfer (gopher://iubio.bio.indiana.edu)
Using glyoxalated RNA
· RNA Electrophoresis (Molecular Genetics RecLab)
· RNA Electrophoresis in Denaturing Formaldehyde Gel (Molecular Genetics Rec Lab)
· Formaldehyde-Agarose Gels (Beverly Faulkner-Jones)
Agarose gels have a smaller resolving power than polyacrylamide gels but a greater range of separation - from 200 bp to >50 kb using standard gels and electrophoresis equipment. RNAs up to 10 000 kb can be separated in agarose gels using pulsed field gel electrophoresis.
· Denaturing polyacrylamide gel electrophoresis of RNA (Beverly Faulkner-Jones)
Polyacrylamide gels have enough resolving power to separate fragments differing by only one base pair in size, but their range is ~ 5 to 1000 bp. They are much more difficult to handle than agarose gels.
· Recovery of RNA fragments from polyacrylamide gels (Beverly Faulkner-Jones)
The best method is the crush and soak method. It can be sued to isolate single-stranded RNA from denaturing gels. The RNA is of high purity but the method is long and inefficient if long transcripts are to be recovered (cf. less than 30% of DNA greater than 3 kb is recovered).
Polyacrylamide Gel Electrophoresis (PAGE) of RNA (Frank Bottone)
For use withRibonuclease Protection Assay (RPA):