| Compound | 15 ml | ||
| MgSO4 | 3.70 g | ||
| H2O | to 15 ml |
| Compound | MW | Final concentration | 1000ml |
| Trypticase Peptone (BBL) | 1 % | 10 g | |
| Beef Extract (Difco) | 0.3 % | 3 g | |
| Yeast Extract | 0.5 % | 5g | |
| K2HPO4 | 174 | 11.5 mM | 2 g |
| NH4Acetate | 77 | 39 mM | 3 g |
| tri-Na-Citrate x 2H2O | 294 | 10.2 mM | 3 g |
| MgSO4 1 M | 246 | 0.8 mM | 0.8 ml |
| MnSO4 0.4 M | 0.4 mM | 1 ml | |
| Tween 80 | 0.1 % | 1 ml | |
| Adjust pH 6.3 with 5N HCl | |||
| H2O, Autoclave | to 1000 ml | ||
| Glucose 50% (or Lactose = MRSL) | 1 % | 20 ml |
| Compound | 50 ml | ||
| NaAc x 3H2O | 17 g | ||
| H2O | to 35 ml | ||
| Adjust pH 5.2 with Acetic Acid | ca. 10 ml | ||
| H2O, Autoclave | to 50 ml |
| Compound | 50 ml | ||
| Na-citrate x 2H2O | 17 g | ||
| H2O | to 50 ml |
| Compound | 50 ml | ||
| NaCl | 14.6 g | ||
| H2O | to 50 ml |
| Compound | 30 ml | ||
| Deoxiycholic acid (Na salt) | 4 g | ||
| H2O | to 20 ml | ||
| Adjust pH 8.4 with 10 M NaOH | |||
| H2O, Filter sterilize | to 30 ml |
| Compound | 500 ml | ||
| NaOH | 200 g | ||
| H2O | to 500 ml |
| Compound | 10 ml | ||
| Tris-Cl 1 M pH 7.2 | 2 ml | ||
| MgSO4 1 M | 400 ml | ||
| DTT 1 M | 4 ml | ||
| H2O | to 10 ml |
| Compound | 1.5 ml | ||
| NiSO4 | 232 mg | ||
| H2O | to 1.5 ml |
| Compound | 500 ml | ||
| Na2HPO4 x 2H2O | 5.33 g | ||
| Bacto Yeast Extract | 2.5 g | ||
| Bacto Peptone | 5 g | ||
| H2O, Autoclave | to 490 ml | ||
| Glucose 50 % | 10 ml |
| Compound | 15 ml | ||
| Guanidine-HCl (GuHCL) | 8.6 g | ||
| Acetic Acid 98 % (~17 M) | 175 ml | ||
| H2O | to 15 ml |
| Compound | 10 ml | ||
| Nitrotetrazolium blue | 200 mg | ||
| Dimethylsulfoxide | 5 ml | ||
| H2O | to 10 ml |
| Compound | 80 ml | ||
| dATP 100 mM | 10 ml | ||
| dGTP 100 mM | 10 ml | ||
| dCTP 100 mM | 10 ml | ||
| dTTP 100 mM | 10 ml | ||
| H2O | 40 ml |