SilaneandPolyLLysineCoatingofMicroscopeSlides

1) Wash slides in Decon solution for 30 minutes.

2) Wash slides in running tap water for 30 minutes.

3) Wash slides in distilled water for 2 x 5 minutes.

4) Wash slides in 95% ethanol for 2 x 5 minutes.

5) Air dry in a dust free environment for 5 minutes.

Poly-L-Lysine Coating

• a) Smear 20ul of poly-l-lysine over each slide.

 


• b) Air dry in a dust free environment.

Silated Slides

• a) To 300mls acetone add 6mls Aminopropyltriethoxysilane solution (Sigma A-3648).

 


• b) Dip slides into silane solution for 10 seconds.

 


• c) Wash 2 x briefly in distilled water.

 


• d) Dry overnight at 37°C in a dust-free environment.