StepsintheGlycosylationofMammalianNlinkedOligosaccarides

The biosynthesis pathway of N-glycans is a costly system with respect to the number of enzymes that are involved in the synthesis and trimming of N-glycans. While the structural diversity of N-glycans in mature proteins in the cell surface is introduced by glycosyltransferases in the Golgi complex, the core glycans in the ER are universal from yeast to mammal and have been considered as intermediates.

Contributor: Kosi Gramatikoff, PhD

REFERENCES: P Burda and M Aebi. The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta, Jan 1999; 1426(2): 239-57. René Cacan and André Verbert. Transport of free and N-linked oligomannoside species across the rough endoplasmic reticulum membranes Glycobiology, Jul 2000; 10: 645 - 648. Robert G. Spiro. Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds Glycobiology, Apr 2002; 12: 43 - 56. Stephanie Grünewald et al. Congenital Disorders of Glycosylation Pediatr. Res., Oct 2002; 52: 618 - 624. Yukiko Yoshida. A Novel Role for N-Glycans in the ERAD System J. Biochem. (Tokyo), Aug 2003; 134: 183 - 190.