抗体纯化

Antibody Purificatioin

• Purification of IgG Using Protein A- or Protein G-Agarose (KPL)
   

• Purifying Antibodies (Perkin-Elmer)

Precipitation Methods
Protein A or Protein G Columns or Beads
Antigen Columns

·         Affinity Purification of Antibodies from Crude Serum(PMCI)

·         Affinity Purification of Antibody (Walter Steffen)

• Coupling procedure

• Affinity purification

• Absorption of antibodies to acetone powder

• Production of acetone powder

• Extraction of antibodies from egg yolk

• Purification and cleavage of murine monoclonal antibodies

• IgG purification with protein G

• Digest of IgG with papain
    

·         Affinity Purification of Antibodies (Vesicle Trafficking)

·         Affinity Purification of Antibodies (XMMR)
protocol in great detail, including column preparation, wash and more...

·         Protein G Purification of Antibodies (Contributed by Nanci Donacki) 

·         Protein A Purification of Antibody (Contributed by Nanci Donacki) 

• Coupling Antibodies to Protein A/G (Breeden Lab)
    

• Coupling Peptides to Resin for Affinity Purification (Mitshison Lab)
    

• Purification of Anti Peptide Antibody (Mitshison Lab) 
      

• Antibody Purification (Mike A. Dyer) 
  This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.

Column Preparation

• Making Affinity Columns (XMMR)