Native gel electrophoresis
Under native PAGE conditions, polypeptides retain their higher-order structure and often retain enzymatic activity and interaction with other polypeptides. The migration of proteins depends on many factors, including size, shape, and native charge. The resolution of nondenaturing electrophoresis is generally not as high as that of SDS-PAGE, but the technique is useful when the native structure or enzymatic activity of a protein must be assayed following electrophoresis. One straightforward approach to native electrophoresis is to omit the SDS and reducing agent (DTT) from the standard Laemmli SDS protocol. Prepare the gels as described above, but do not put SDS or DTT in the sample buffer, do not heat the samples, and prepare the gel and tank buffer solutions without SDS.
尺寸排阻色谱(SEC)与非变性电喷雾电离质谱(NativeESI-MS)联用技术是研究天然蛋白质的有效工具。但已报道的研究大多集中于技术应用,并未验证蛋白质在所用分离条件下是否处于原始状态,也未评估S......
分析测试百科网讯2019年4月29日,由上海药物研究所和布鲁克公司共同举办的“InternationalSeminarontheStateofArtinNativeMassSpectrometry”研......
当前,光通讯和化合物半导体相关行业中,GelPak的 AD系列凝胶盒和 VR系列真空释放盒得到了越来越多用户的认可.对于胶盒产品,胶的稳定性是最关键的指标,稳定性表现在以下几个方面......