二、细胞实验效果不错，那动物实验怎么做？
稳定性、重复性较好的动物模型可在肿瘤基础性研究和抗肿瘤药物筛选中发挥重要作用。血液肿瘤常用的动物模型主要有：I皮下移植瘤模型；II血液模型。皮下移植瘤模型较容易建立，易于动态观察肿瘤的生长状态。血液模型则一般以生存天数作为观察终点，用活体成像、病理切片、流式细胞术等方法检测小鼠骨髓及脏器中的肿瘤细胞生长及浸润情况。该模型与白血病患者的病理生理状况较为接近，但实验难度较大。
三、体内外模型选好了，那么我的实验需要使用哪种慢病毒载体对基因进行操作呢？
启动子、荧光标记、抗性标签是我们选择载体需要考虑的三大要素。实验不同，这些元件也需要相应变化。按例祭出神图一张，定制你的专属载体，就是这么简单！
上海吉凯基因化学技术有限公司成立于2002年，拥有BSL-2级别的慢病毒包装实验室。病毒生产线通过ISO9001质量管理体系验证，月均定制基因病毒产品包装超过1000次。慢病毒生产采用6项QC检测、病毒纯度分级纯化以及病毒滴度绝对定量检测确保病毒质量。
400名兢兢业业的吉凯人，用专业换您的高效。
PS：希望了解更多肿瘤工具指南的朋友们，可以关注“吉凯基因”微信公众号，更多精彩文章等您哦。
参考文献
【1】 T Zhou et al. “Downregulation of Mcl-1 through inhibition of translation contributes to benzyl isothiocyanate induced cell cycle arrest and apoptosis in human leukemia cells.” Cell Death and Disease (2013) 4, e515.
【2】 Xin Huang, et al. “Analysis of the expression of PHTF1 and related genes in acute lymphoblastic leukemia .” Cancer Cell International 2015 15:93.
【3】Wang, Yi, et al. "Downregulation of miR-3940-5p promotes T-cell activity by targeting the cytokine receptor IL–2R gamma on human cutaneous T-cell lines." Immunobiology 221.12 (2016): 1378-1381.
【4】Jian Zhang, et al. “ SUMOylation of insulin-like growth factor 1 receptor, promotes proliferation in acute myeloid leukemia.” Cancer Letters , 2015, 297–306.
【5】Zhen-Hua Chen, et al. “The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway .” Cell Death and Differentiation (2016), 1–13.
【6】 Xiuxiu Liu, et al. " Elevated AEG-1 expression in macrophages promotes hypopharyngeal cancer invasion through the STAT3-MMP-9 signaling pathway." ncotarget (2016): 12886.
【7】Guo, Jiang-Rui, et al. "Hepatocyte growth factor promotes proliferation, invasion, and metastasis of myeloid leukemia cells through PI3K-AKT and MAPK/ERK signaling pathway." American journal of translational research 8.9 (2016): 3630.
【8】Lixian Wu, et al. “Dual Inhibition of Bcr-Abl and Hsp90 by C086 Potently Inhibits the Proliferation of Imatinib-Resistant CML Cells.” Clinical Cancer Research 2015.
【9】 Xiaobing Miao, et al. “Overexpression of TRIP6 promotes tumor proliferation and reverses cell adhesion-mediated drug resistance (CAM-DR) via regulating nuclear p27Kip1 expression in non-Hodgkin’s lymphoma.” Tumor Biology 2016, 37, 1, 1369–1378.
【10】Bihui Yang, et al. “SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis.” International Journal of Oncology, 2014, 649-656.
【11】Armen Mardiros, et al. “T cells expressing CD123-specific chimeric antigen receptors exhibit specific cytolytic effector functions and antitumor effects against human acute myeloid leukemia.” BLOOD, 31 . 2013 .122.18.