PREPARATIONOF2％FORMALDEHYDESTOCKSOLUTION(2METHODS)
METHOD 1:Formaldehyde preservative – 2% formaldehyde solution in protein-free phosphate-buffered saline (PBS).Prepare as follows:Add 2 g paraformaldehyde powder (e.g., Sigma, St. Louis, MO) to 100 ml of 1 X PBS. Heat to 70°C (do not exceed this temperature) in a fume hood until the paraformaldehyde goes into solution (note that this happens quickly as soon as the suspension reaches 70°C). Allow the solution to cool t......阅读全文
PREPARATION OF 2% FORMALDEHYDE STOCK SOLUTION (2 METHODS)
METHOD 1:Formaldehyde preservative – 2% formaldehyde solution in protein-free phosphate-buffered saline (PBS).Prepare as follows:Add 2 g paraformaldeh
Preparing Antibiotics Stock Solution and Ampicillin Agar Plates
AMPICILLIN Beta-lactam-antibiotics are not very stable when dissolved. Slow but steady degradation happens even when frozen to -20°C. Therefore comme
常用试剂配制－2
Acetone / Formaldehyde FixativeAcrylamide Gel, denaturingAcrylamide Gel, nondenaturingAlkaline Phosphatase Buffer 1 (Glycine Buffer, 0.1 M, pH 10.4)Al
Fluorescence In Situ Hybridization using TSA™
实验概要This protocol describes steps for fluorescent in situ hybridization (FISH) to Drosophila embryos using Tyramide Signal Amplification (TSA™), and
Measurement of GFP Expression and DNA Content in Permeabilized Cells
Reagents Cells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as a control. 1 X PBS
FISH protocols for Drosophila-1
.1 RNA Probe Preparation (see Note 1)1. 1.5 mL microcentrifuge tubes or standard 96-well V-bottom microplates.2. RNAse free water.3. T7, T3 or S
ASENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
Immunohistochemistry on paraffin embedded sections
主要试剂1. Neutral-buffered Formalin, 10% (NBF), 1 liter (Commercially available pre-prepared from many laboratory reagent suppliers).Double -distilled H
3 Color Staining: Alpha-Catenin in HeLa Human Cervical Cancer Cells
实验概要Alpha-catenin in HeLa human cervical cancer cells was labeled using mouse anti-α-catenin and visualized with Alexa Fluor® 488 goat anti-mouse Ig
In-Vitro Adipocytes Differentiation
Introduction Obesity is a significant clinical problem that contributes to life-threatening diseases such as diabetes and atherosclerosis. With an i