WIKI资讯
WIKI资讯
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ field.Introduction:The axolotl (Ambystoma mexicanum) is a large salamander which is native to Lake Xochimilco, Mexico. The axolotl is a member of a larger group of salamanders known as mole salamanders. Other members of this group include tiger salamanders and spotted sala......阅读全文
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the mor
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
实验概要During more than 25 years of application in immunological sciences, ELISPOT has been established as a routine, robust, versatile, and
In addition, proficiency panel projects have demonstrated that even after an experiment was done and spots were counted, considerable vari
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location of th
DefinitionAmino acids, nucleotides, polypeptides, and other compounds in a colloidal state can be separated by the application of external voltages wh
if mouse is found deadif mouse is a newborn or < 1 wk of ageIf mouse is alive or moribund and above one week of ageRoutine dissectionFormalin fixat
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
IntroductionLive imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It all
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the
Isolation of DNA from filamentous fungi for PCR analysis is usually time consuming and involves use of toxic chemicals such as phenol/chloroform. In&n
Protocol for the Primary Culture of Cortical and Hippocampal neurons Solutions and media required:Poly D-lysine/laminin solution - pdfD
实验概要The CellTrace™ CFSE Cell Proliferation Kit provides a versatile and well-retained cell-tracing reagent in a convenient and easy-to-use
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Cor
2012年7月5日，美国食品与药物管理局（FDA）批准了首个巨细胞病毒（CMV）DNA检测法，用于评估实质器官移植患者的抗病毒治疗是否有效。在患者正在接受抗巨细胞病毒治疗期间，医师可以借助该方法评估治疗过程中患者血清样本中CMV载量变化情况。如果两次检测期间病毒载量显著降低则说明治疗有效，反之则应该
美国当地时间2月8日，美国工程院公布2017年入选院士名单，共新增84位院士及22位外籍院士。其中有多位著名华人学者，包括黄永刚、沈向洋、张东晓、丛京生、陈向力等人。 美国国家工程院院士是工程界最高荣誉之一，授予那些在工程领域内从事研究、实践和教育并作出卓越贡献的人士；以及在新兴和发展中的技
BCEIA2019 International Summit on Analytical Instrumentation and In Vitro Diagnosis--Registration in ProgressIn recent years，In Vitro Diagnosis has be
实验概要Cytokine ELISPOT has become a powerful routine tool for the analysis of disease- as well as vaccine-induced T-cell responses. The meth
Introduction: This method for the detection of cellular proliferation includes several modifications of a previously published protocol (Hayashi, et a
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
Introduction介绍Theoretical Framework理论概况Granular materials and fine powders are widely used in industrial applications. To control and to optimize pr
Adrenal chromaffin granule (chromaffin vesicle) preparationIntroduction. This prep is adapted from the classic paper of Smith and Winkler (Smith
OTA (Over The Air) OTA stands for Over The Air. In order to perform test a device with any test equipment, you need a way of connecting the d
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
五、使用仪器发表文章AuthorDateTitleJournalCell TypeCellometer / ApplicationsMahato, Ram INovember 2013Synthesis and Characterization of an Anti-Apoptotic Immu
The major naturally occurring auxin, indol-3 acetic acid (IAA), coordinates many growth and differentiation processes by modulating gene expressio