RegulativeDevelopmentinAxolotlEmbryos;SplittingtheHeartField
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic field that is responsible for heart formation using grafted tissue from the gill area, and attempt to form two hearts – one on either side of the grafted tissue.BackgroundOrganisms that use regulative development rely on cell-cell interactions to determine wh......阅读全文
Regulative Development in Axolotl Embryos; Splitting the Heart Field
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic
Development of the Heart Morphogenetic Field in the Axolotl Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Axolotl Protocol: Transplantation of the Gill Organ Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
Developmental and phenotypic implications of transplantation
Objective In this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate
Axolotl Microsurgery
I. Embryo Preparation1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embryo
NFAT and Hypertrophy of the heart (Transcription in the broken heart)
Hypertrophy associated with both hypertension and obstruction to ventricular outflow leads to pathologic cardiac growth and it is associated with incr
Routine Splitting and freezing of cells
1. Grow cells to subconfluence in a flask. 2. Harvest as per normal and count. 3. Spin down 5min 1.2K in benchtop. Resuspend at 1.0 X 106/ml in 10%
Pulse Field Electrophoresis
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
Dark Field Viewing
Dark Field ViewingPrincipleTo view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scatte
In VitroCulture of Chicken Heart
The chicken is a classic organism used to illustrate the principles of basic embryology. One of the developmental systems which has been examined in g