WIKI资讯
WIKI资讯
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimensional (2D) gel electrophoresis has provided a tool for differential protein display, which allows for the quantitative analysis of many (~500-1000) of proteins simultaneously. Complex biological questions can now be approached by analyzing differences in the 2D gel pattern......阅读全文
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension
ABSTRACTFor MALDI-TOF-MS analysis of proteins and peptides, samples are cocrystallized with an excess of organic matrix that absorbs at a specific wav
ResultsIdentified novel royal jelly proteins To expand the number of known proteins in the RJ proteome, RJ proteins were extracted and digeste
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
Preparation of protein samplesIntracellular virus proteinsThe following method has been developed principally for the analysis of intracellular protei
生物分子发表的代表性文献 QTRAP：同时具有三重四极杆和线性离子阱性能的独一无二的LC/MS/MS系统 QTRAP系统最早在ASMS 2002上，作为第一台商用的线性离子阱发布，是世界上唯一的线性离子阱和三重四极杆的复合
Promega利用ProteaseMAX表面活性剂推出了一项创新简化的胶内酶切技术。该项技术给分析人员提供了以下几点好处： 节省时间：整个胶内酶切过程只需一个小时的时间 省去了萃取步骤：蛋白质分解和肽段回收一步完成。 更多的数据：该分解技术保持了原有肽段的信息。
尊敬的各位同行： 第2 届大连国际色谱学术报告会及仪器展览会包含第37届国际高效液相色谱及相关技术会议(含仪器展览会) (HPLC 2011 Dalian) 及第18届全国色谱学术报告会及仪器展览会(18th NSEC)，将于2011年10月8-11日在大连召开。 为使更多色谱工作者
Comprehensive identification of novel proteins and N-glycosylation sites in royal jellyLan Zhang1,2†, Bin Han1†, Rongli Li1, Xiaoshan Lu1,3, Aiying Ni
RapiGest SF虽然是一种表面活性剂，但其全面适应液质分析的要求。在使用RapiGest SF作为变性剂完成蛋白酶切后，如果只进行色谱分析，则可无需对RapiGest SF进行处理。如进行液质联用分析，则通过简单的加酸步骤就可将RapiGest SF去除。而酸性溶液环境又恰恰是液质
与其他的蛋白酶合用 我们测试了 RapiGest SF 与多种蛋白酶的适配性，如 Asp-N, Lys-C 与 Glu-C 。在酶解前使用 RapiGest SF 变性蛋白获得了有效的消解结果。蛋白去糖基化的用途 RapiGest SF 也用于测试其它酶，如 PNGase F ，该酶用于酶切糖蛋白
在生物药肽图分析、蛋白质修饰分析、蛋白质组学研究等诸多工作中，蛋白质的变性与酶解是样品处理的必经之路。RapiGestTM SF正是在这个实验步骤中，用以辅助蛋白酶解的变性试剂[1,2,3]。RapiGest SF有以下优点：■ 综合水相、有机相溶液条件下，最高效的辅助酶解变性剂。■ 作用
N-glycosylation modification of proteins has reported to improve the health of living organisms through antibacterial activity [68], antioxidant a
INTRODUCTIONOne versatile strategy for sample cleanup prior to MALDI-MS analysis uses microscale columns designed for direct sample elution onto the M
1,关于使用三氯醋酸沉淀法后蛋白质难于重悬的问题Q:1,Hi !I have been trying to clean and concentrate proteins form eucariotic cell culture medium in order to analyse them in 2
RJ provides efficient energetic fuels for the fast development of larvae and the egg-laying queen through the metabolism of sugars, lipids, and pr
1. Excision of protein bands (spots) from polyacrylamide gelsRinse the gloves you use with water to avoid traces of dust in your sample.Rinse the gel
· Designing Your Peptide (Genosys)· &n
粒径和UPLC/HPLC的放大性 许多肽分离操作仍在传统的HPLC仪器上进行。因其背压过高，使用亚2 μm填充的色谱柱通常不适合与HPLC系统联用。而2.5 μm色谱柱因其背压较低可以在任何LC仪器上使用。为确定CSH130 C18,1.7μm色谱柱得到的峰容量是否可以成功转移到CSH130 C18
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
Experimental sectionChemicals and reagentsOne lot of PuregonR-HP of 50 IU/0.5mL and two lots of PuregonR-HP of 100 IU/0.5mL (rhFSH) (Organon, Oss, N
梁宋平简介： 梁宋平，1970年毕业于北京大学生物学系，1983年获北京大学生物化学硕士学位（导师李建武教授），1986年获北大生物化学博士学位（导师张龙翔教授）。1986-1990年在美国波士顿大学进行博士后研究。1990年至现在在湖南师范大学生命科学学院从事教学与科研工作，1992年晋
基于此，CSH130 C18和BEH130 C18生成最佳峰形的流动相条件应该有所不同。为此，还使用10倍酸性的酸(1%HOAc)调节的流动相进行了分离。尽管中等浓度对于开发纯化工艺可能具有一定参考价值，但此处未对其进行评估。如图3所示，改变流动相组分后，CSH柱的峰形大大改善，但BEH柱的峰形变得
诺华（Novartis）制药 Wang, Karen（王瑛琪）教授 来自诺华（Novartis）制药的Wang, Karen（王瑛琪）教授，做了题为“Application of Mass Spectrometry in Pharmaceutical Drug Discovery Research
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (r
As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of
Take the appropriate set of Fmoc-amino acid stock aliquots for cycle 1 from the freezer, bring to room temperature, and activate by adding DIC (4 µl p
实验概要The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently