WIKI资讯
WIKI资讯
DescriptionFor protein concentration, gas pressure is applied directly to ultrafiltration cell. Solutes above the membrane's molecular weight (MW) cut-off are retained in cell, while water and solutes below the cut-off pass into the filtrate and out of cell. 80508400Cell capacity (ml) Stirred minimum volume¹ (ml) Membrane diameter (mm) Effective membrane area (cm ²) Hold-up volume² ......阅读全文
DNA PreparationE. coli chromosomal DNA is prepared following the method of Heath et al. ( J. Bacteriol., 174, 1992). Cells are embedded in agarose, th
作者：刘凤珍，邢家林，柳薇，龚庆成 【摘要】 目的 观察体外循环预充的库存悬浮红细胞(悬红)经超滤后电解质及生化指标的变化。方法 将作为小婴儿预充的悬红2个单位为1份，共选30份悬红，入选悬红存放日期为5～13 d。30份悬红超滤前为A组(n=30)，超滤后为B组(n=30)，
Preparing Lambda DNA1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required
I. TYPES OF CELLS GROWN IN CULTURETissue culture is often a generic term that refers to both organ culture and cell culture and the terms are often us
Purification of Kar3 Motor Domain ProteinMaterialsInduced cells (2 - 5 g pellet of pET/Kar3 in BL31(DE3)pLysS host cells) (See note #1)HEM b
RNA interference (RNAi) is a phenomenon in which the introduction of double-stranded RNA (dsRNA) into a diverse range of organisms and cell types caus
1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were confirmed by clini
Phycoerythrin conjugation protocolDavid's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal an
目前，胎牛血清（Fetal Bovine Serum, FBS）是细胞培养实验中最常使用的试剂，为细胞生长提供丰富的营养物质。但在外泌体研究中，如果仍然使用胎牛血清培养细胞、通过收集上清液来提取外泌体进行实验，胎牛血清中大量的牛源外泌体将对实验分析造成极大的干扰。因此，使用FBS时，必须要做牛源外泌
目前，胎牛血清（Fetal Bovine Serum, FBS）是细胞培养实验中最常使用的试剂，为细胞生长提供丰富的营养物质。但在外泌体研究中，如果仍然使用胎牛血清培养细胞、通过收集上清液来提取外泌体进行实验，胎牛血清中大量的牛源外泌体将对实验分析造成极大的干扰。因此，使用FBS时，必须要做牛源外泌
GST Fusion Protein PrepItalics indicate optional steps especially useful for the analysis of untested proteins.GROWTH AND HARVESTING OF BACTERIAAdd 10
Objective: This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the extern
This workbook was developed for use with Module 2 of the InVitro Insights Cell Culture Training Program, developed by Becton Dickinson. The
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zúñiga-Pfl
人胚肾 293 (HEK293) 细胞在重组蛋白表达中是最常见的宿主细胞。 这类细胞能够表达大量的膜蛋白，如 G 蛋白偶联受体 (GPCR) ，是无法在最常见的生物制药生产宿主，如：中国仓鼠卵巢 (CHO) 细胞中作表达。 HEK293 虽然是蛋白表达的极好宿主，然而 H
Freezing ESCs 25. Passage the ESCs as described in Step 22. Resuspend the cells in 3 mL of freezing medium for OP9 cells. 26. Aliquot&n
TROUBLESHOOTINGProblem: The OP9 cells are more than 80%-90% confluent.Solution: It is important when creating working stocks of OP9 cel
实验概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
Seeding After cells are thawed:NOTE: Do not dispense the entire contents of the cryovial into one T-25 flask!!Remove the cap, being car
ProcedureI. INTRODUCTIONThe 293 EBNA cell line is established from primary embryonal human kidney cells transformed with sheared human adenovirus type
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
REFERENCES:R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach s
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
Protocol 3: Fetal Liver-Derived HSC Differentiation on OP9-DL1 CellsDay 0: Initiation of Fetal Liver Co-culture52. Isolate liver tissue from eight to
MediaHigh glucose DMEM (-pyruvate, -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type, be sure!!)1X l-glutamine1X Penicillin/stre
常规操作(主要内容如下)· Aseptic Technique· Culture Ves
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
Care and Handling of Feeder Layer CellsSTO/SNL cells are a derivative of the standard STO cell line. They are transformed with a LIF producing plasmid
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
实验概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the