Purificationofpoly(A)+RNAfrom&nb
0. (Optional)Before using Oligotex-dT30, I recommend to wash Oligotex-dT30 to remove fine particle of latex. To wash the latex, transfer appropriate amount of Oligotex-dT30 (300 ul of the suspension of Oligotex per 1mg of total RNA) into a microfuge tube. Spin for 3 min at 12,000 rpm. Discard the supernatant. Gently suspend the latex in the same volume of 10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 0.1% SDS. Spin again. Gent......阅读全文
Purification of poly(A)+ RNA from&nb
0. (Optional) Before using Oligotex-dT30, I recommend to wash Oligotex-dT30 to remove fine particle of latex. To wash the latex, transfer appropr
RNA Collection & Purification from fibrous tissue
实验概要 Provides an easy and fast method for isolating total RNA from fibrous tissues which contains contractile proteins, connective tissue and co
Sauer:RNA Purification from E. coli
My Experience Purifying RNA from E. coliRegarding RNA extraction, there is a horrible tendency of people to use kits for RNA extraction with bacteria.
RNA Purification from 10-20 mg Paraffin-embedded Tissue
实验概要 The E.Z.N.A.® SQ Tissue RNA Kit is designed for isolating total RNA from animal tissue and cultured cells. The solution based system can be e
Antibody Purification
This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography. 1. Solutions (1) Affigel Blue Prewash 0.1
Purification of Demethylated Sphingomyelin
I. Lower, chloroform phase: 1) Dry on rotovapor system with house vacuum lines. It is not necessary to dry sample completely, but sufficiently to yi
Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buff
Purification of mAb (IgG)
1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.
Protein purification; actin
Protein purification; actin Overview ACTINThe most abundant muscle and non-muscle cytoskeletal protein. MW 42 kDa, 374/375 amino acids; various
Column Purification of Demethylated Sphingomyelin
Packing column: 1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of chloroform. 2) Place a small portion of glass wool at the base of the