WIKI资讯
WIKI资讯
实验概要The Dynabeads® and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface of Dynabeads, and b) use the antibody coated beads for co-immunoprecipitation. This kit is intended to be used for the co-immunoprecipitation of proteins, intact protein complexes, intact protein-nucleic acid complexes, etc. The antibody coated ......阅读全文
实验概要This protocol is intended for activation and expansion of human Treg cells isolated with the Dynal® CD4 CD25 Treg Kit (Cat. no. 113.23D). The exp
实验概要Protein G, a cell wall component produced by group G Streptococcus strains, binds the Fc part of a wide range of immunoglobulins (Ig’s
实验概要Intended UseDynabeads® Streptavidin are ideal for numerous applications, including purification of proteins, nucleic acids purificatio
实验概要Intended UseDynabeads® Streptavidin are ideal for numerous applications, including purification of proteins, nucleic acids purificatio
实验概要The Dynabeads and buffers provided in this kit will enable you to covalently immobilize antibodies (or other protein ligands such as &
实验概要Dynabeads® Streptavidin are ideal for numerous applications, including purification of proteins, nucleic acids purification, protein i
实验概要The Dynabeads® and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
3. General Immobilization Protocol Wash the Dynabeads® before use.
实验概要Human embryonic stem cells can be differentiated into neural-, mesenchymal and hematopoetic stem cells. This product is intended for t
实验概要Enrich untouched Dendritic Cells (DCs) by depleting T cells, B cells, monocytes/ macrophages, NK cells, erythrocytes and most granuloc
人肿瘤抗原可以：（1）用于肿瘤诊断；（2）免疫治疗。实验方法原理 本实验用Dynabeads protein A 进行免疫复合物的纯化。Dynabeads A 蛋白 （Dynabeads Protein A） 在免疫沉淀中有以下作用：（1）将方案时间减少到 30 分钟。（2）显著降低非特异
实验概要The CellTrace™ Violet Cell Proliferation Kit provides a versatile and well-retained cell tracing reagent in a convenient and easy-to-u
MATERIALSMedia:Heat inactivated FBS (56°C x 30 min)PBS + 2% heat-inactivated FBSIMDM + 20% heat-inactivated FBSViral transduction: Iscove's +
实验方法原理 实验材料 感兴趣的细胞或组织试剂、试剂盒 糖原EDTA缓冲液SDSBSATween 20连接子T4 DNA 连接酶BsmFIPC8SeeDNA乙酸钠乙醇DMSOPCR引物乙酸铵DNA ladder 聚丙烯酰胺 TBE凝胶DNA参照pZErO-1 质粒TE缓冲SOC培养液Tris · C
实验材料 肿瘤细胞试剂、试剂盒 PBS生理盐水裂解液蛋白酶抑制剂仪器、耗材 培养瓶细胞刮棒离心管低温离心机-80℃冰箱探针型超声波恒温水浴锅实验步骤 一、 裂解细胞1. 方向刮取细胞。将细胞悬液转移到离心管，离心取沉淀，-80℃ 保存。（收集细胞要迅速，低温下进行，以减弱蛋白酶的作用）2
当找不到适合自己的细胞类型的阳性分离试剂盒时该怎么办？最佳的选择是将Dynabeads™ FlowComp™ Flexi试剂盒与一款用于细胞分离的合适靶标抗体联用。Flexi试剂盒含有进行分离和释放操作所需的部分试剂，包括用于对您的抗体进行生物素化操作的DSB-X生物素化试剂盒，经修饰的链霉亲和
This method was submitted by Jim Hutchins from the University of Mississippi Medical Center.The following is a great RT- PCR protocol develo
This method was submitted by Jim Hutchins from the University of Mississippi Medical Center.The following is a great RT-PCR protocol developed by
Isolation of mice glomeruli1. Mice were anesthetized by an intraperitoneal injection of Avertin (2,2,2-tribromoethyl and tertiary amyl alcoho
实验材料感兴趣的细胞或组织试剂、试剂盒糖原EDTA缓冲液SDSBSATween 20连接子T4 DNA 连接酶BsmFIPC8SeeDNA乙酸钠乙醇DMSOPCR引物乙酸铵DNA ladder聚丙烯酰胺 TBE凝胶DNA参照pZErO-1 质粒TE缓冲SOC培养液Tris · Cl仪器、耗材微量离心
作者：Xiaojuan Liu1, *, Yongping Zhang2, *, Chen Cheng1, 3, *, Albert W Cheng4, Xingying Zhang1, 5, Na Li1, Changqing Xia2, 6, Xi
试剂、试剂盒 10 mol L ATP PNK (9.3 U ul) 5 x T4 DNA 连接酶缓冲液 LoTE
主要内容如下：· RT-PCR· Competitive and Quantative
试剂、试剂盒 10 mol L ATPPNK (9.3 U ul)5 x T4 DNA 连接酶缓冲液LoTE实验步骤 1.稀释引物IB和2B, 至质量浓度为350ng/V。2.加入下列试剂进行引物5’末端磷酸化：3.在37℃ 下孵育30 min。4.在65℃ 下放置 10 min热失活PNKo5.将
-免疫学家和肿瘤学家正在利用过继细胞转移技术，操纵人体的免疫系统来抵抗癌症和其他疾病。在正常的免疫反应中，一种被称作T细胞的白细胞经另一种被称作抗原呈递细胞（APC）的免疫细胞的指导后扩大它们的数量，并且保持存活。过继细胞转移程序正是在培养皿中模拟这一过程，即从患者身上取出T细胞，让它们繁殖，有
·样本制备：所有的分离操作都必须在单细胞悬液中进行。·请确保使用了正确的细胞数量，稀释倍数和抗体混合物体积，Dynabeads™磁珠和缓冲液系统。·在混匀步骤使用HulaMixer™样品混合器（目录编号15920D）以确保分离操作中的混合效果良好。·在接触磁力架前的最后操作步骤中，用户须使用1 mL
这一步仅为基因表达的系列分析 cDNA 合成中实验方案 A 的一部分，在实验方案 B 中，cDNA 已经结合到链霉抗生物素包被的 PCR 试管上，因此不必用磁珠结合。实验材料磁珠试剂、试剂盒Dynabead M-280链霉抗生物素仪器、耗材磁设备实验步骤实验方案 A振荡 lmin 以使 Dynabe
美国生命技术公司(Life Technologies)7月31日宣布，已与诺华(Novartis)签署了一项长期的供应及独家许可协议，Life Technologies将提供其专有技术——磁珠CD3/CD28 CTS，用于诺华抗癌免疫疗法的开发，涉及修饰T细胞表达嵌合抗原受体，用于癌症治
试剂、试剂盒 溶液与缓冲液 LoTE 仪器、耗材 Epp
试剂、试剂盒 溶液与缓冲液LoTE仪器、耗材 Eppendorf 试管实验步骤 实验方案 A1.向含有 Dynabeads 的每个试管中加入下列物质：2.65℃ 下酶解 Ih(每 IOmin 混合 1 次)。3.时收集上清液（含有释放的 cDNA 标签）4.用 LoTE 扩容到 200mL。5.等体