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PhosphoaminoacidanalysisofnonTCAprecipitableproteins

Phosphoamino acid analysis of non-TCA precipitable proteins1. Cut out gel slice, incubate 60 min with 1 ml 5.7 M HCl @ 110oC in 13 x 100 mm screw cap tubes.2. Add 4 ml water, centrifuge @ 2000 rpm in bench top centrifuge for 10 min, transfer supernatant, freeze and lyophilise.3. Dissolve residue in 10 ml water, add 0.4 ml 50% (v/v) slurry of Dowex AG 1 X 8 (Cl- form), adjust pH to 7.5 - 8.5 with NH4OH.4. Add 1 µl of ......阅读全文