CellThawing／CellFreezingProtocol
Freezing Cells:Cells should be growing well or known to be in log phaseCount, collect and pellet cells in a 15mL test tubeResuspend in freezing media so that the concentration is no more than 5x10^6 cells/mL of cold freezing mediaTransfer 1mL of cells to appropriately labelled cryovials and maintain on ice for approximately 30minutesTransfer vials to -80C freezer for 24hrsTransfer to liquid nitrogen dewar or -140C fr......阅读全文
Cell Thawing/Cell Freezing Protocol
Freezing Cells: Cells should be growing well or known to be in log phase Count, collect and pellet cells in a 15mL test tube Resuspend in fr
Cell Thawing/Cell Freezing Protocol
Freezing Cells:Cells should be growing well or known to be in log phaseCount, collect and pellet cells in a 15mL test tubeResuspend in freezing media
Freezing and Thawing of Mammalian Cell Lines
For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture.
ES and TS cell freezing/thawing
Needed: ES cell freezing medium (2x) 2x ES cell freezing medium should be made up fresh each time it is to be used, and should comprise freshly pre
ES and TS cell freezing/thawing
实验概要ES and TS cell freezing/thawing.主要试剂ES cell freezing medium (2x) 2x ES cell freezing medium should be made up fresh each time it is to be
Protocol for Cell Fusion
Healthy Sp2/0 cells should be rapidly growing by this time. Sp 2/0 cells should be started about two weeks before the cell fusion. Every two days, the
Cell Extraction Protocol
实验概要Primary tissues are valuable tools for the study of intracellular and extracellular markers which characterize disease states. We have developed
stem cell culture protocol
实验概要stem cell culture protocol主要试剂cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
T cell Activation Protocol
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
PrestoBlue™ Cell Viability Reagent Protocol
实验概要PrestoBlue™ Cell Viability Reagent is a ready‐to‐use reagent for rapidly evaluating the viability and proliferation of a wide range of cell type