WIKI资讯
WIKI资讯
1) Aliquot 5 X 106 total cells in 5-8 ml 2% media.2) Add 30 µl 3H-thymidine.3) Incubate for approximately 16 hours under normal growth conditions.4) Spin down cells and wash 2-3 times with PBS (or serum free media) to remove any unincorporated label.5) Resuspend cells at 5 X 105/ml in serum free media.6) Aliquot approximately 400 µl of cell suspension into each well of a 24 well plate.7) Treat cells (ALWAYS HAVE TIME......阅读全文
hoechst33258染色，他们认为细胞发生凋亡时，染色质会固缩。 所以Hoechst染色时，细胞核会呈致密浓染，或呈碎块状致密浓染。正常细胞核刺激后有致密浓染的凋亡细胞 AO-EB染色法：The image below shows human lymphoma cells
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
Viable cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells i
3.6 Key references1. Kroemer G., Zamzani N., Susin S.A. Mitochondrial control of apoptosis. Immunol. Today, 18: 44-51, 1997.2. Susin S.A., Z
实验概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apopto
作者：杨连君，司晓辉，王文亮，王文勇，赵一岭，方正清［摘 要］ 目的：探讨简便易行的在光镜下通过形态学观察检测细胞凋亡的方法，并对其进行比较。方法：首先用6%的乙醇作用6 h诱导人肝细胞癌细胞系HCC9204细胞凋亡，然后进行未固定细胞的台盼蓝染色、吖啶橙/溴化乙啶(AO/EB)双染色，
Mammalian cells can respond to a variety of stresses such as heat, cold, oxidative stress, metabolic disturbance, and environmental toxins through nec
Mitochondria participate in apoptotic signaling pathways through the release of mitochondrial proteins into the cytoplasm. Cytochrome c, a key protein
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
ASTRACTWe describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell
2.3 凋亡细胞的TUNEL和ISNT鉴定的流式细胞仪分析[16]对于培养的细胞，可以将TUNEL或ISNT鉴定同流式细胞仪结合起来分析其发生凋亡的情况。待检细胞与含有TdT或DNA聚合酶I或Klenow片段及生物素标记的dUTP反应液共孵育一段时间后，加入荧光素（常用FITC）标记的链霉抗生物
3 胆汁淤滞性肝病在许多临床综合征中可以观察到胆汁淤滞，胆汁淤滞是一组慢性进行肝病的主要特征，并可最终导致肝硬化、肝衰竭和死亡。肝内疏水的毒性胆盐储滞长期以来被认为是肝损伤的一个主要原因，并被认为是胆汁淤滞性肝病肝损伤的一个关键原因。事实上肝内毒性胆盐：鹅脱氧胆酸和脱氧胆酸盐的储滞水平与肝损伤的程度
Figure 1.Determination of apoptosis in transiently transfected murine [beta] tumor cells. (A) The number of apoptotic [beta]HC 13T tumor cells (%
3. Commentary 3.1. Background informationApoptosis is an innate mechanism of eukariotic cell suicide which plays a major role i
Chapter 1: Cell Death - Apoptosis and Necrosis1.1Introduction21.1.1Terminology of cell death21.1.2Differences between necrosis and apoptosis31.1.3Apop
1. INTRODUCTION Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pat
Chemical Induction of Apoptosis - 1 May 2001p53, p21WAF1, Myc, Bcl-2, Bax, Bcl-x and bak are among the proteins involved in the regulation of apoptosi
Apoptotic cell death can be triggered by many different cellular stimuli, resulting in activation of apoptotic signaling pathways including caspases (
Apoptosis is specifically induced via signaling through a family of receptors known collectively as 'death receptors' including Fas, TNFR, DR3
原文检索： Type 3 inositol 1,4,5-trisphosphate receptor negatively regulates apoptosis during mouse embryonic stem cell differentiation Ca2+ sign
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
IntroductionWhen studying induction of apoptosis via a cell surface molecule, it is important to first ascertain surface expression of the molecule of
实验概要Provides a rapid and convenient assay for apoptosis. 实验原理Apoptosis is a carefully regulated process of cell death that occurs as a norm
Programmed cell death is induced by many different factors and involves numerous signaling pathways, some dependent on caspase proteases and others th
One mechanism used by cytotoxic T cells to kill tumor cells and virus-infected cells is the release of perforin and granzyme proteins. Perforin protei
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
Expression of Fas-Ligand by T cells can induce apoptosis through interaction with Fas on the target cell. A target of Fas-mediated apoptosis is the B
THE CELL 1. Morphological aspects of apoptosis Walter Malorni, Stefano Fais & Carla Fiorentini 2. Cell cycle Miriam
Receptors in the TNF receptor family are associated with the induction of apoptosis, as well as inflammatory signaling. The Fas receptor (CD95) mediat
The PML nuclear bodies are ring-shaped nuclear substructures associated with the regulation of transcription, transformation, cell growth, and apoptos