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IsolationandQuantificationofGenomicDNAfromMycobacteriumtuberculosis

Part A. Isolation of Nucleic AcidsNOTE: CAUTION! STEPS 1-10 SHOULD BE PERFORMED USING APPROPRIATE PROCEDURES FOR HANDLING MATERIAL POTENTIALLY CONTAMINATED WITH MYCOBACTERIUM TUBERCULOSIS. ALSO,THE LIDS OF THE EPPENDORF TUBES SHOULD BE CAREFULLY CLOSED AND OPENED SO AS TO AVOID SPLASHES AND AEROSOL =46ORMATION.1. Using a 1ml plastic, disposable pipet attached to a Pipet-Aid motorized pipettor, add 1 ml sterile TE buf......阅读全文