WIKI资讯
WIKI资讯
PCR:We generally use 1-2 ul of starting paraffin microdissected DNA for each 50 ul DOP-PCR reaction.We assume that about 1 ug of product is produced in each DOP-PCR reaction. The entire product is then nick translated as described below.Nick Translation of the PCR Product:For the first CGH, our approach is to start with predefined test and reference labels, and then to do a replicate ("inverse") reaction ba......阅读全文
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number