WIKI资讯
WIKI资讯
一、毛细管电泳的概述：毛细管电泳又称高效毛细管电泳，它是在熔融的石英毛细管（内径为25~100m）中进行电泳，其管内填充缓冲液或凝胶，是近年来进展最快的分析方法之一。毛细管电泳是电泳技术和现代微柱分离相结合的产物，它具有效率更高、速度更快、样品和试剂消耗量特少的特性。毛细管电泳仪的基本结构：1、 高压电极槽与进样机构 2、填灌清洗机构 3、毛细管 4、检测器 5、铂丝电极 6、低压电极槽 7、恒温机构 8、记录/数据处理毛细管的结构：毛细管电泳通常使用内径为25~100μm的弹性（聚酰亚胺）涂层熔融石英管。 毛细管的特点是：容积小；侧面/截面积大而散热快、可承受高电场；可使用自由溶液......阅读全文
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
DNA电泳（主要内容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA fro
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. DNA is a negatively
10 Fun Facts for DNA Electrophoresis::Migration of DNA is retarded and band distortion can occur when too much buffer covers the gel. The slower migra
Section 2.1Nondenaturing Polyacrylamide Gel Electrophoresis of ProteinsJohn M. Walker1. IntroductionSDS-PAGE (Section 2.2) is probably the most
Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to h
C. Restriction digestionRestriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restrict
· RNA Gel (Crawford Lab)· Gel Electroph
DefinitionAmino acids, nucleotides, polypeptides, and other compounds in a colloidal state can be separated by the application of external voltages wh
蛋白质电泳（主要内容如下）One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel Gel StainingRecipesOne-Dimensional SDS-PAGE·&
INTRODUCTION After chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is p
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
A. Preparation of DNA SolutionIn the case of rice, for example This method may be appllicable for many grass species and some other plants. &n
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location of th
Lipoprotein Analysis Week 2: Electrophoresis IntroductionSDS polyacrylamide gel electrophoresis (SDS PAGE) will be used to assess the
RNA Gel (Crawford Lab)Gel Electrophoresis of RNA (Beverly Faulkner-Jones)great tips on RNA gel electrophoresis.Northern Gel and TransferUsing gl
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
毛细管电泳（Capillary Electrophoresis，CE），又称高效毛细管电泳，是一类以毛细管为分离通道、高压直流电场为驱动力的新型液相分离分析技术 (Fig.1)。自20世纪90年代以来，该技术迅速被各种标准 (包括中国药典、国标、美国药典，甚至是欧洲标准) 所收录，成为现代分析科学中
Serum Protein Electrophoresis Tricine/Polyacrylamide Gel ElectrophoresisUsed for pilin processing analysis but generally useful for resolution of
③蛋白质分析 Duffy等［16］利用CD盘式塑料阵列芯片采用离心的方式进行了碱性磷酸酶分析，每个样品检测只需3mL试剂，几分钟内可分析几十个样品。瑞典的GYROS公司已生产出类似的产品并进行了肌球蛋白、IgG、IgA分析［17］。近来Burke 和Regnier［18］在芯片上利用电
摘要 通过毛细管微乳液电动色谱10 min内同时分离了安非他明、甲基安非他明、4,5亚甲基二氧基安非他明(MDA)和3甲氧基4,5亚甲基二氧基安非他明(MDMA)4种苯丙胺类毒品及其麻黄碱、伪麻黄碱、甲基麻黄碱、甲基伪麻黄碱、去甲麻黄碱等麻黄生物碱杂质。比较了毛细管微乳液电动色
Assay of superoxide dismutase activity by combining electrophoresis and densitometryAbstract. A modified technique was developed to assay superoxide d
1. 前言提取蛋白质是蛋白质组学研究的第一步。植物组织的蛋白质含量较低，并且存在多种非蛋白质成分，如细胞壁及贮藏多糖、脂质和酚类化合物，使蛋白质的提取难度增大 。植物蛋白质的可溶性与它们的细胞内定位关系密切，传统的蛋白质提取方法是用水合缓冲液、去垢剂或直接沉淀法 [1] 。除了普遍使用的 TCA
使用毛细管等电聚焦分析5型AV的稳定性已被描述[17] 。然而，这是首次被描述的使用电泳滴定曲线测定完整病毒颗粒的电荷行为。 也采用ETC对经320nm紫外线照射15分钟灭活的麻疹病毒进行了分析。琼脂糖凝胶电泳曲线证实在PH值低于7时病毒带正电荷，
1. IntroductionNuclear morphology changes characteristic of apoptosis appear within the cell together with a distinctive biochemical event: the endonu
METHOD Analysis of precipitated chromatin fractions (from Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analy
1.2 亲和层析(Affinity Chromatography,AC) AC是利用连接在固定相基质上的配基与可以和其特异性产生作用的配体之间的特异亲合性而分离物质的层析方法。自1968年Cuatrecasas提出亲和层析概念以来，在寻找特异亲和作用物质上发现了许多组合，如抗原-抗体、酶-催化底物
Alkaline agarose gel electrophoresis (Sambrook et al., 1989)Alkaline agarose gels can be used to determine the size and quality of first and second st
实验概要RNA analysis on non-denaturing agarose gel electrophoresis实验步骤1. The following gel electrophoresis conditions are recommended:- use 1X TAE buffer
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over