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QuickYeastDNAPrep:IsolationofTotalDNA(genomicandplasmid)

Grow a 5 ml YPD O/N culture inoculated with a single yeast colony at 30 deg.Transfer culture to a small 13 x 100 glass tube. Spin down cells 2 min. in tabletop centrifuge. Pour off supernatant.Wash cells with 5 ml H2O. Spin down cells 2 min. Pour off supernatant. Cell pellet can be stored at -20 deg.Resuspend cells in 500 ul lysis buffer by vortexing.Lysis buffer:20 ml:0.1M Tris pH 8.02 ml 1M Tris 8.050 mM EDTA2 ml 0......阅读全文