ManipulatingExpressionofTonoplastTransporters
Plant vacuoles have multifaceted roles including turgor maintenance, cytosolic pH and ionic homeostasis, plant protection against environmental stress, detoxification, pigmentation, and cellular signaling. These roles are achieved through the coordinated activities of many proteins in the tonoplast (vacuolar membrane), of which the proton pumps and ion transporters have been modified for improved abiotic ......阅读全文
Manipulating Expression of Tonoplast Transporters
Plant vacuoles have multifaceted roles including turgor maintenance, cytosolic pH and ionic homeostasis, plant protection against environmental st
Protein Expression and Purification Protocol
Step 1: Transform appropriate DNA plasmid into BL21(DE3) E. coli cells. These cells must be competent. (Protocol for how to make competent cells.)a) T
Measurement of Green Fluorescent Protein Expression
Reagents Cells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as control. Hoechst
FOSB gene expression and drug abuse
Drug addiction is associated with long-term behavioral changes, suggesting a long-lived transcriptional regulator that responds to chronic drug exposu
Bacterial Expression of GST-fusion Proteins
1. Grow cells in 5ml (or more) of LB-Amp overnight for a starter culture. 2. Grow larger culture (100x volume of starter culture) using the ove
MicroRNA Expression Profiling by Bead Array -3
Table 2 Modulation of microRNA expression by IFNα—4 and 24 h after stimulation Control Interferon-alpha treated
MicroRNA Expression Profiling by Bead Array -4
Bead-array-based microRNA detection technology, including the bio-statistic analysis, is currently not well established or widely used and we have app
Atlas™cDNA表达距阵(Expression Array)
AtlasTM cDNA表达距阵(Expression Array)同时对588种/1176种关键基因做全景表达图谱分析同时对大量已知基因的表达进行大规模高通量(High-htroughput)分析检测关键基因在细胞关键功能中的角色只需简单的杂交步骤就在数年前，核酸矩阵技术只能在资金雄厚的大实验室开
Double Stranded RNA Induced Gene Expression
One defense against viral infection is provided by PKR, double-stranded RNA activated protein kinase. When PKR interacts with dsRNA found in cells dur
Control of Gene Expression by Vitamin D Receptor
The vitamin D receptor, VDR is the mediator of all genomic actions of vitamin D3 and its analogs. It belongs to a family of ligand induced transcripti