Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting messenger RNA (mRNA) stability and inhibiting mRNA translation. The expression of miRNA species in cellular populations is thought to drive downstream gene expression and protein functionality. Our goal was to determine the variability in miRNA expre......阅读全文
结论· 我们在C1TM单细胞自动制备系统开发了一种简洁的实验方案,能以最少的手工操作,在不到24小时内,平行处理高达96个单细胞,对其miRNA表达谱进行分析。· C1 miRNA STA实验方案使用了Life Technologies为miRNA优化过的试剂。特别的,Meg