ELISA:Direct
实验概要The following protocol provides a method of direct ELISA by Peprotech.主要试剂Tween-20 (Sigma Cat. # P-7949) BSA (Sigma Cat. # A-7030) ABTS Liquid Substrate Solution (Sigma Cat. # A3219) Dulbecco’s PBS [10x] (Gibco BRL Cat. #14200-075) All solutions should be at ambient temperature prior to use. PBS: Dilute 10xPBS to 1xPBS, pH 7.20 in sterile water. Wash Buffer: 0.05% Tween-20 in PBS Block Buffer: ......阅读全文
ELISA: Direct
实验概要The following protocol provides a method of direct ELISA by Peprotech.主要试剂Tween-20 (Sigma Cat. # P-7949) BSA (Sigma Cat. # A-7030) ABTS Liquid Su
Direct/Indirect Staining Protocol
Use this Protocol for Directly or Indirectly staining cells for Flow Cytometric Analysis 1) Dilute cells to 5x10^6 cells/mL 2) Aliquot 100uL of ce
Direct PCR from Whole Yeast Cells: Zymolyase Method
Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pel
DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES
I. PurposeTo identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleuke
全血直接PCR试剂盒Blood Direct PCR Kit
全血直接PCR试剂盒Blood Direct PCR Kit全血扩增引物设计注意事项1）引物3’端zui后一个碱基选择C或G；2）引物3’端zui后8个碱基应避免出现连续错配；3）引物3’端尽量避免出现发夹结构；4）引物Tm值控制在60℃-72℃之间 (推荐使用软件Primer Premier 5进
ELISA
Gangliosides ELISA protocol (Contributed by pingsunjim)This protocol can be used for detection of gangliosides.Specific antibodies to gangliosides are
Competitive ELISA
DAY 11. Coat Nunc immuno-module plates overnight, in usual manner.2. Set up competition assay between antibody and competing substance :-Prepare a 1:2
ELISA直接法和间接法优缺点
直接法（direct ELISA）将抗原直接固定在固相载体上，加入酶标记的一级抗体，即可测定抗原总量，此一级抗体的特异性非常重要。优势：操作手续简短，因无须使用二抗可避免交互反应。缺点：试验中的一抗都得用酶标记，但不是每种抗体都适合做标记，费用相对提高。间接法（indirect ELISA）此测定方
乙酰化蛋白快速检测和定量
Rapid detection and quantitation of total cellular acetylated proteins using our research products
ELISA
ELISA是一种经典的测定液体样本中蛋白含量的方法。该方法优点在于可以准确定量蛋白含量，且测定样本的种类可以多种多样。本期小编带你一起了解ELISA不同样品的制备方法。细胞培养上清液移取细胞培养基至离心管中，4℃ 下以 1500 rpm 的速度离心 10 分钟。立即分装上清液，并将样品储存在 -80