牛粒细胞巨噬细胞集落刺激因子／BovineGMCSF重组蛋白中英文说明书

艾美捷牛粒细胞-巨噬细胞集落刺激因子/Bovine GM-CSF重组蛋白中文说明书：

目录编号（规格）：

RP0871B-005（5µg）

RP0871B-025（25µg）

RP0871B-100（100µg）

来源：酵母

别名：CSF-2

配方：无载体蛋白冷冻。

重建：用至少含有磷酸盐的无菌缓冲盐水重建

0.1%载体蛋白。

稳定性和储存：在-20°C温度下，自收到之日起可稳定12个月。在工作等分试样中储存时至少可稳定3个月在-20°C下具有载体蛋白。避免反复冻融循环。

分子量：14.3 kDa（计算）

生物活性：重组牛GM-CSF的生物活性为在使用人TF-1的细胞增殖测定中测量细胞系。这种效应的ED50通常为2.5-4.0 ng/mL。

纯度：通过SDS-PAGE分析显示>95%。

纯化：离子交换色谱法

Entrez基因ID:281095

氨基酸序列：APTRPNTAT RPWQHVDAIK EALSLLNHSS DTDAVNDTE VVSEKFDSQE ptclctrlkl YKNGLQGSLT SLMGSLTMMA

氨基酸同源性：牛头（牛）GM-CSF–100%

Bos mutus（野牦牛）GM-CSF–99%

野牛野牛（野牛）GM-CSF–98%

印度博斯（泽布）GM-CSF–98%

原产国：美国

牛粒细胞-巨噬细胞集落刺激因子/Bovine GM-CSF重组蛋白英文说明书：

Catalog Number (Quantity): RP0871B-005 (5 µg)

RP0871B-025 (25 µg)

RP0871B-100 (100 µg)

Source: Yeast

Alias: CSF-2

Formulation: Lyophilized without carrier protein.

Reconstitution: Reconstitute with sterile phosphatebuffered saline containing at least

0.1% carrier protein.

©01/18/2019

Stability and Storage: Stable for up to twelve months from date of receipt at -20°C.

Stable for at least 3 months when stored in working aliquots

with a carrier protein at -20°C. Avoid repeated freeze/thaw

cycles.

Molecular Weight: 14.3 kDa (calculated)

Bioactivity: The biological activity of recombinant bovine GM-CSF was

measured in a cell proliferation assay using the human TF-1

cell line. The ED50 for this effect is typically 2.5 - 4.0 ng/mL.

Purity: >95% as visualized by SDS-PAGE analysis.

Purification: Ion-exchange chromatography

Entrez Gene ID: 281095

Amino Acid Sequence: APTRPPNTAT RPWQHVDAIK EALSLLNHSS DTDAVMNDTE

VVSEKFDSQE PTCLQTRLKL YKNGLQGSLT SLMGSLTMMA

THYEKHCPPT PETSCGTQFI SFKNFKEDLK EFLFIIPFDC

WEPAQK (126)

Amino Acid Homology: Bos taurus (cattle) GM-CSF – 100%

Bos mutus (wild yak) GM-CSF – 99%

Bison bison bison (bison) GM-CSF – 98%

Bos indicus (zebu) GM-CSF – 98%

Country of Origin: USA

Warranty: Products are warranted by Kingfisher Biotech, Inc. to meet stated product

specifications and to conform. to label descriptions when used, handled and

stored according to instructions. Unless otherwise stated, this warranty is

limited to one year from date of sale or expiry date, whichever comes first.

Kingfisher Biotech’s sole liability for the product is limited to replacement of

the product or refund of the purchase price. Kingfisher Biotech products are

supplied for research applications. They are not intended for medicinal,

diagnostic or therapeutic use.

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