蛋白质标记

• Biosynthetic labeling (Sefton Lab)

• Biotinylation of Antibody (Contributed by Nanci Donacki)

• ¹²⁵I Labeling of Protein using ICl (ScienceXchange)

• Protein (Antibody) Fluorescent Labeling (Mario Roederer)
  Gives detailed protocol for conjugation of proteins to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V). Procedures for the conjugation of immunoglobulins are provided for the following molecules:

• Fluorescein (FITC)

• Phycoerythrin (PE)

• Cy5PE

• Cy7PE

• Texas Red (TR)

• Allophycocyanin (APC)

• Cy5

• Cy7APC

• Cascade Blue

• Biotin

In addition, protocols for conjugation of Annexin V to the following molecules are given as models for fluorescent conjugation of non-immunoglobulin proteins. 

• FITC

• PE

• TR-BSA

• APC

• Cascade Blue