质谱学报纪念杨芃原教授专辑

杨芃原教授是我国质谱领域的先锋科学家，他充沛的探索热情和无私的奉献精神影响了一代又一代质谱人。

杨芃原教授热心中文期刊发展事业，他曾担任《质谱学报》第六、七、八、九、十届副主编，还亲自为学报策划“质谱仪器研制专辑”。

为传承杨芃原教授的学术精神，《质谱学报》编辑部于2026年5月出版了“纪念杨芃原教授专辑”。

感谢执行主编周振教授、陆豪杰教授在专辑的策划、组约稿件等方面给予的帮助，感谢各位老师为该专辑提供优质稿件。

综述

化学酶法标记整合质谱技术在蛋白质糖基化分析中的研究进展

赵晨阳,李中华,陆豪杰

DOI: 10.7538/zpxb.2025.0154

This review highlighted recent advances in chemoenzymatic labeling-assisted mass spectrometry for glycoproteomics, emphasizing innovative strategies that integrate enzymatic specificity with chemical versatility to enable selective enrichment, site-resolved identification, and high-throughput analysis of complex protein glycosylation networks.

基于质谱的临床体液糖蛋白质组学研究进展

蔡飞,令颖颖,李玲,申靖,张勇

DOI: 10.7538/zpxb.2025.0155

This review summarized recent advances in mass spectrometry-based glycoproteomics of human body fluids, highlighting disease-specific glycosylation alterations and their potential as biomarkers for clinical translation.

黏蛋白型O-糖基化蛋白质组学分析进展

刘兆亮, 于永亮, 叶明亮

DOI: 10.7538/zpxb.2025.0156

This review systematically summarized recent advances in mucin-type glycopeptide enrichment—including novel chromatographic materials, O-glycoprotease-based techniques, and bioorthogonal approaches—and mass spectrometry (MS)-based database search strategies. These advances enable quick comprehension of cutting-edge progress and provides valuable insights for developing innovative methodologies.

基于质谱的单细胞蛋白质组学研究进展

付航, 王璐璐, 翟琳辉, 谭敏佳

DOI: 10.7538/zpxb.2025.0150

This review comprehensively summarized advances in mass spectrometry-based single-cell proteomics, highlighting innovations in automated isolation, nanoliter-scale sample preparation, and data analysis. It further explored emerging spatial proteomics technologies and clinical applications, providing critical insights into future multi-omics integration and standardization.

生物组织完整蛋白原位电离与空间组学质谱技术的进展和挑战

杨治渝, 杨婷婷, 王娜, 岳磊

DOI: 10.7538/zpxb.2025.0169

This work highlighted in situ mass spectrometry strategies for intact protein analysis by integrating in situ sampling and ionization with gas-phase separation and top-down mass spectrometry. The combined IMS-top-down-MSI approach enables structural characterization of membrane proteins, protein complexes, and low-abundance proteins with spatial resolution.

基于质谱的免疫肽组学在肿瘤新抗原鉴定中的研究进展

杨雨岱, 余利岩, 徐平, 张振鹏

DOI: 10.7538/zpxb.2025.0148

This review summarized recent methodological advances in mass spectrometry (MS)-based immunopeptidomics for tumor neoantigen identification, highlighting innovations in sample preparation, data acquisition, and database construction, and emphasizing how integrated multi-omics and advanced MS technologies enable more reliable and experimentally grounded neoantigen discovery.

人工智能助力蛋白质翻译后修饰的质谱解析及其临床应用

谢欣然, 杨晓霞, 申华莉, 林灵

DOI: 10.7538/zpxb.2025.0119

This study outlined how artificial intelligence (AI) bridges key technical challenges in post-translational modification (PTM) research to enable clinical translation. The analytical bottlenecks were summarized, including low sensitivity and coverage, structural heterogeneity, and site ambiguity, as exemplified in PTMs such as phosphorylation, glycosylation, acetylation, and lactylation. And the corresponding AI-driven computational strategies were highlighted, encompassing deep learning models, algorithm development, and dedicated PTM data-processing pipelines. The resulting translational outcomes were illustrated, including advances in cancer diagnosis, mechanistic dissection of disease pathways, and the discovery of novel therapeutic targets.

肿瘤与非经典蛋白：从“暗蛋白质组”到诊疗新策略

范子鸿, 王苏杰, 何庆瑜, 张静

DOI: 10.7538/zpxb.2025.0139

This study systematically illustrated the research framework of the dark proteome, delineating the biological origins of noncanonical proteins, the multi-omics technologies for their discovery, their multifaceted roles in tumor biology, and their clinical translational potential.

人参属植物药效成分的直接质谱分析研究进展

胡莘如, 刘春颖, 眭沁雪, 张小平, 陈焕文

DOI: 10.7538/zpxb.2025.0144

The review summarized direct ionization techniques (e.g., DART, EESI) coupled with mass spectrometry for rapid, in situ ginseng analysis within 1-60 s without sample pretreatment. This method enables high-throughput, real-time characterization of bioactive compounds, thereby driving advancements in traditional herbal medicine quality control and pharmacological research.

研究论文

四极杆离子碰撞诱导解离系统的研制及其参数优化

朱晨鑫, 刘凯, 杨芃原, 申华莉

DOI: 10.7538/zpxb.2025.0122

This work innovatively developed a dual-mode (transient/static voltage) CID system on a self-built platform, establishing a systematic parameter optimization workflow. The static voltage mode doubled MS/MS signal intensity and enabled closed-loop optimization, thus enhancing domestic instrument capabilities and providing a new strategy for high-performance tandem mass spectrometry.

同轴反射式微型飞行时间质谱仪的研制

杨栋, 刘瑞东, 吴向坤, 李静, 侯可勇

DOI: 10.7538/zpxb.2025.0149

In this work, a coaxial reflection miniaturized time-of-flight mass analyzer was designed. Its overall performance and related influencing factors were optimized, enabling the detection of copper, iron, aluminum, titanium, and nickel.

超低温离子阱-红外光解离光谱-飞行时间质谱的研制及水合离子团簇的结构和温度依赖性研究

池超贤, 王柏纯, 金佳晔, 王冠军, 周鸣飞,

徐福兴, 丁传凡

DOI: 10.7538/zpxb.2025.0180

A home-built temperature-controlled cryogenic ion trap mass spectrometer coupled with IRPD spectroscopy was used to probe hydrated metal ion clusters and reveal how temperature alters isomer populations and hydrogen-bonding motifs.

大气压气动雾化-飞行时间质谱仪研制及应用

张振元, 蒋吉春, 樊志刚, 任美慧, 黄天浩,花磊, 李海洋

DOI: 10.7538/zpxb.2025.0162

In this study, a TOF-MS equipped with a high-efficiency octopole atmospheric pressure interface was developed. It provides high sensitivity across a broad m/z range of 15-650 and a LOD of 0.3 μg/L for reserpine. The instrument holds significant potential for microdroplet research and in situ characterization of battery electrolytes when coupled with pneumatic spray ionization.

基于timsTOF Pro2的非标记单细胞蛋白质组学技术优化

陈丽颖, 范林园, 张跃飞, 黄玉滢, 李军,

徐宁, 任艳, 刘斯奇

DOI: 10.7538/zpxb.2025.0164

This study optimized a label-free single-cell proteomics workflow using a timsTOF Pro2 mass spectrometry. Through systematic optimization of sample preparation, LC-MS parameters, and DIA settings, deep proteome coverage (~2 459 proteins/cell) with high reproducibility was achieved. This study offers a cost-effective strategy for laboratories equipped with comparable instrumentation.

面向亲和富集-质谱分析通用需求的生物信息学分析平台

张骞宁, 何岸, 李元, 柯弥, 田瑞军

DOI: 10.7538/zpxb.2026.0028

APMSflow is an integrated R pipeline that transforms complicated AP-MS data processing into a user-friendly workflow. By removing the need for extensive custom coding, it uniquely unifies steps from quality control through to interaction network prediction, thereby streamlining protein-protein interaction analysis for experimental researchers.

棕榈酸诱导HepG2细胞脂毒性模型的深度蛋白质组学

郭佳鸣, 陈光云, 谢文凡, 徐亦阳, 王宇秋,王诗盛, 王贺瑶, 朱洪文, 周虎

DOI: 10.7538/zpxb.2025.0158

This study presented a high-depth proteomic profile of palmitic acid-induced lipotoxicity in HepG2 cells. By integrating three sample preparation methods, the robust protein alterations were identified and the novel mechanisms involving transcriptional regulation, organelle dysfunction, and calcium homeostasis were revealed.

二维离子迁移谱串联质谱用于探索蛋白质结构多样性

吴凯群, 雷永琪, 王陈璐, 李俊晖, 俞建成,唐科奇

DOI: 10.7538/zpxb.2025.0141

Multiple IMS peaks can be observed in the IMS spectra for myoglobin ions with +12 to +14 charge states, while multiple FAIMS peaks can be observed in the FAIMS spectra for myoglobin ions with +15 to +25 charge states. This indicated that the gas phase ion separations in FAIMS and IMS are highly orthogonal.

高脂血症患者血脂净化治疗的血清蛋白质组学研究

徐东, 杨叶晓青, 刘容, 胡中瀚, 吕定丰,唐科奇

DOI: 10.7538/zpxb.2025.0143

LC-MS/MS combined with data-independent acquisition analysis was used to explore the effect of lipoprotein apheresis in patients with hyperlipidemia. Differential expression and bioinformatic analyses were further performed to screen for the potential target proteins which may directly or indirectly prevent or mitigate the progression of hyperlipidemia.

基于质子转移反应质谱快速分析纺织品中挥发性有机物

李丹丹, 何智豪, 李成林, 江小琴, 段忆翔,赵忠俊

DOI: 10.7538/zpxb.2025.0140

This study developed a rapid PTR-MS method for VOCs screening in textiles, eliminating sample preparation. It achieves detection within one minute per sample and low detection limits, enabling high-throughput analysis for large-scale quality control.

基于激光烧蚀碳纤维离子化质谱法快速鉴别化妆品包装材料

郑世博, 郑荣, 孟茜, 戴维, 苏越,郭寅龙

DOI: 10.7538/zpxb.2025.0146

This study introduced a rapid, in situ LACFI-MS method for authenticating cosmetic packaging. It directly analyzes surface chemistry without sample pretreatment, identifying stable fingerprints for genuine products and disordered signals for counterfeits, enabling efficient discrimination and potential source tracing.

甲醇介导的多膜集成过滤装置用于多组学样品的快速制备

闫帅, 崔春萍, 秦伟捷, 张万军

DOI: 10.7538/zpxb.2025.0137

In this study, a method of methanol-mediated multi-Omics via filter (3M-Filter) was introduced, which enables rapid, parallel preparation of proteomics and metabolomics samples within 30 min. This unified workflow provides high-depth and highly reproducible multi-omics coverage from diverse and low-input samples, facilitating robust LC-MS analysis.