WIKI资讯
WIKI资讯
General GuidelinessiRNA targeted sequence is usually 21 nt in length.Avoid regions within 50-100 bp of the start codon and the termination codonAvoid intron regionsAvoid stretches of 4 or more bases such as AAAA, CCCCAvoid regions with GC content <30% or > 60%.Avoid repeats and low complex sequenceAvoid single nucleotide polymorphism (SNP) sitesPerform BLAST homology search to avoid off-target effects on other ......阅读全文
The siRNA user guide (revised May 6, 2004) Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysa
Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed t
RNAi target selection rules:Targeted regions on the cDNA sequence of a targeted gene should be located 50-100 nt downstream of the start codon (ATG).S