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CreationandUseofInfectiousVirusVector

Creation and use of your infectious vector:Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired).The following day set up (use polypropylene tubes for this; polystyrene tubes DO NOTwork!).1 ug retroviral DNA encoding gene X1 ug packaging plasmid (such as pCLEco, pCLampho, pUMVC, pHR’CVM8.2 deltaR etc.). If you are using a 3 plasmid system then:for lenti: 1 μg packa......阅读全文